Specific Antibody Production in Plant Cell Cultures

本研究的目标是开发一种大规模的过程，利用修饰的植物细胞来生产功能性哺乳动物抗体蛋白。转化的植物组织培养细胞具有合成功能性抗体蛋白的能力，在廉价、高效地生产大量特异性抗体方面具有很大的潜力。单域抗溶菌酶抗体cdna已被亚克隆到植物表达载体中，并被引入到培养的植物细胞中，用于生产抗体蛋白。将测试各种信号肽序列，以便从培养细胞中有效地分泌抗体蛋白到培养基中。进行细胞培养的规模化和优化。将监测产生抗体的细胞的遗传稳定性。

The goal of this proposed research is to develop a large-scale process to produce functional mammalian antibody proteins by employing modified plant cells. The creation of transformed plant tissue culture cells capable of synthesizing functional antibody proteins has great potential for producing large quantities of specific antibodies cheaply and efficiently. Single domain anti-lysozyme antibody cDNAs have been subcloned into a plant expression vector and introduced into cultured plant cells which will be employed for the production of antibody proteins. Various signal peptide sequences will be tested for the efficient secretion of antibody proteins into the medium from cultured cells. The scale-up and optimization of the cell cultivation will be carried out. The genetic stability of the antibody-producing cells will be monitored.