Understanding Injury of Suspended Animal Cells in Mixed Bioreactors Due to Bubble Breakup

为了向密集的动物培养细胞群提供氧气和其他营养物质，搅拌和充气是必要的。了解流体-机械力对脆弱动物细胞的损伤对于在不同规模的生物反应器中培养这些细胞并用于各种应用是一个重要的问题。首席调查员(PI)建议调查由于气泡破裂而影响细胞所经历的力的因素。采用鼓泡塔和搅拌生物反应器研究操作参数和培养基组分对细胞损伤的影响。视频显微镜将被用来可视化和量化细胞与气泡相互作用的某些方面。PI还建议研究Sparger和叶轮设计，使生物反应器中的细胞能够喷雾和混合。

Agitation and air-sparging are necessary for providing oxygen and other nutrients to dense populations of cultured animal cells. Understanding the injury of fragile animal cells due to fluid-mechanical forces is an important issue to the culturing of these cells in bioreactors of various scales and for various applications. The Principal Investigator (PI) proposes to investigate the factors that effect the forces experienced by cells due to bubble break-up. Bubble-column and agitated bioreactors will be used to study the effect of operational parameters and medium composition on cell injury. Video microscopy will be employed to visualize and quantitate some aspects of cell to bubble interactions. The PI also proposes to investigate sparger and impeller designs that will allow the sparging and mixing of cells in bioreactors.