An Analysis of Hybrid Zone Dynamics in Iris

这项研究的目的是确定杂交的效果 属于同一物种的个体之间以及 在基因上属于两个不同物种的个体 鸢尾属杂交种群的结构和进化。 亲子 分析（使用蛋白质和DNA标记）将用于辨别 自然产生的杂交体中的基因交换模式 人口和在一个I。fulva种群，其中I.六角形 个人被介绍。 亲子鉴定也会 允许估计花粉与种子运动对 这两个种群的遗传结构。 这些分析 将用于确定基因流对（i） （二）两个物种之间的基因转移; （三）个别工厂的成功和（三）工厂的数量， 在杂交种群中相互杂交。 的影响 自然选择对I.富尔瓦岛 六角菊属及其自然杂种将通过播种 将来自每个物种的种子和天然杂交种转化为I.fulva， I.六边形和杂交种群。 自然选择在 因此，不同的物种和杂交种将通过记录 发芽率，存活到开花，花数 生产和总的生物质生产。 这些测量将 允许计算I的相对适合度。富尔瓦岛 不同生境类型的六角藻和各种杂交类群。 适合度的估计，扩散的测量和 杂种群体中不同遗传标记的频率 将被用来测试预测特定进化的模型， 混合人口的结果。

The aim of the proposed study is to determine the effect of crosses between individuals belonging to the same species and between individuals belonging to two different species on the genetic structure and evolution of hybrid populations of irises. Paternity analyses (using protein and DNA markers) will be used to discern patterns of gene exchange in a naturally occurring hybrid population and in an I. fulva population where I. hexagona individuals have been introduced. The paternity analysis will also allow an estimate of the impact of pollen versus seed movement on the genetic structure of these two populations. These analyses will be used to determine the affect of gene flow on (i) the transfer of genes between the two species, (ii) the reproductive success of individual plants and (iii) the number of plants that cross with one another in the hybrid populations. The effects of natural selection on the survival and reproduction of I. fulva, I. hexagona and their natural hybrids will be estimated by sowing seeds from each of the species and natural hybrids into I.fulva, I. hexagona and hybrid populations. Natural selection on the different species and hybrids will, thus be measured by recording germination frequencies, survival to flowering, numbers of flowers produced and the overall biomass produced. These measurements will allow the calculation of the relative fitness of I. fulva, I. hexagona and various hybrid classes in different habitat types. The estimates of fitness, measurements of dispersal and the frequencies of different genetic markers in the hybrid populations will then be used to test models that predict specific evolutionary outcomes for hybrid populations.