Acquisition of an Analytical Ultracentrifuge

俄勒冈州立大学生物化学和生物物理系的许多研究项目都依赖于分析沉淀方法的使用。这些研究包括染色质的结构、稳定性和组装的研究（K.E. van Holde）；血青素的结构、进化和功能的研究（K.I. Miller， K.E. van Holde）；参与蛋白质磷酸化的酶之间的关联的研究（S.R. Anderson）；以及参与DNA前体生物合成的多酶复合物（C.K. Mathews）。van Holde博士的实验室多年来一直维护并稳定使用贝克曼e型分析超离心机。这仪器既陈旧又破旧。幸运的是，对于我们未来的研究，贝克曼仪器公司刚刚推出了一种全新的分析超离心机设计。这台仪器不仅可以满足我们系目前的需要，而且可以进行各种迄今不可能进行的实验。本提案要求为这一工具提供资金。作为一个在利用和开发超离心技术方面处于领先地位的集团，我们有能力最大限度地利用这种设施。

A number of research projects in the Department of Biochemistry and Biophysics, Oregon State University, depend on the use of analytical sedimentation methods. These include studies of the structure, stability, and assembly of chromatin (K.E. van Holde): studies of the structure, evolution, and function of hemocyanins (K.I. Miller, K.E. van Holde): investigators of association between enzymes involved in protein phosphorylation (S.R. Anderson); and multi-enzyme complexes involved in biosynthesis of DNA precursors (C.K. Mathews). The laboratory of Dr. van Holde has maintained, and steadily used for many years a Beckman Model E. analytical ultracentrifuge. This instrument is both obsolete and decrepit. Fortunately for our future research, the Beckman Instrument Company has just come out with a radically new analytical ultracentrifuge design. This instrument will not only meet the present needs of our department, but will allow kinds of experiments hitherto impossible. This proposal requests funds for such an instrument. As a group that has been a leader in the utilization and development of ultracentrifuge techniques, we are in a position to make maximum use of such a facility.