Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia

这个项目的目标是在厌氧丁酸梭菌的分子遗传学和酶学方面产生新的知识，使有益的基因在各种应用中细胞新陈代谢的改变。丁酸梭菌在生产日用品和特种化学品方面具有很好的工业应用潜力。目前关于这些细胞的遗传学、调节和新陈代谢的知识基础是有限的，但是，以前的赠款工作已经取得了相当大的进展。本项目将继续基因调控的研究，进一步开发梭状芽胞杆菌的表达系统，并评估参与调节梭状芽孢杆菌代谢途径的其他因素。

The objectives of this project are to produce new knowledge on the molecular genetics and enzymology of anaerobic butyric clostridia that will allow beneficial genetic alterations of the cell metabolism for a variety of applications. Butyric acid clostridia have an excellent potential for industrial applications to produce commodity and specialty chemicals. The current knowledge base on the genetics, regulation and metabolism of these cells has been limited, but considerable progress has been made by work on previous grants. This project will continue the study of gene regulation, develop further the expression systems in clostridia, and evaluate other factors involved in the regulation of metabolic pathways in clostridia.