Neuron-glia Differentiation of a Stem Cell Line; RT4

神经系统由两种主要的细胞类型，神经元和神经胶质细胞组成。本研究的长期目标是阐明神经系统中胶质细胞与神经元细胞身份形成的分子机制。S100B和胶质纤维酸性蛋白两个基因的调控将被研究。这两种基因都在神经胶质细胞中表达，而在神经元细胞中不表达。这些基因将在本实验室从神经源性肿瘤中培养的细胞系中进行研究。目前研究的主要重点是识别和表征这些基因中的控制元件，这些元件负责打开和/或关闭该细胞系中这些蛋白质的产生。这些研究是我们理解神经系统发育的基础

The nervous system is composed of two main cell types, neurons and glia. The long term objective of this research is to elucidate the molecular mechanism responsible for development of the glial versus neuronal cell identity in the nervous system. The regulation of two genes, S100B and Glial Fibrillary Acidic Protein, will be studied. Both of these genes are expressed in glial cells but not in neuronal cells. These genes will be studies in a cell line developed by this laboratory from a tumor of neuronal origin. The primary focus of the current study is to identify and characterize the control elements in these genes that are responsible for turning on and/or off the production of these proteins in this cell line. These studies are fundamental to our understanding of the development of the nervous system.***