Acquisition of a Confocal Laser Microscope

9317664 Angelides本提案要求提供资金购买共聚焦激光扫描显微镜。 该仪器将满足一些教师的研究需要，他们的研究工作重点是活细胞的动力学，亚细胞结构的三维排列，以及完整脑切片中神经元等细胞的三维重建。 除其他专题外，该仪器将用于利用荧光探针和抗体研究脑切片中离子通道和受体的分布;核和有丝分裂结构的组织和动力学;海马体中突触的发育;基因转移过程;表皮中角蛋白的调节和功能;在培养细胞中过度表达的平滑肌钙调蛋白的定位和在肌成束蛋白（一种肌动蛋白结合蛋白）中的定位;肌生成过程中的结蛋白和波形蛋白;以及乳腺癌的激素调节。

9317664 Angelides This proposal requests funds to purchase a confocal laser scanning microscope. The instrument will serve the research needs of a number of faculty whose research work focuses on the dynamics of living cells, the three-dimensional arrangement of subcellular structures, and the three- dimensional reconstruction of cells such as neurons in intact brain slices. Among other topics, the instrument will be used for studies of the distribution of ion channels and receptors in brain slices using fluorescent probes and antibodies; the organization and dynamics of nuclear and mitotic structures; the development of synapses in the hippocampus; gene transfer processes; the regulation and function of keratins in the epidermis; localization of overexpressed smooth muscle caldesmon in cultured cells and localization in fascin, an actin binding protein; desmin and vimentin during myogenesis; and hormonal regulation of breast cancer.