Acquisition of Automated DNA Sequencer for Primate Molecular Evolutionary Research

在美国国家科学基金会的支持下，Maryellen Ruvolo博士将购买一套自动DNA测序系统，包括：1。应用生物系统公司的自动DNA测序仪，带有必要的软件，用于编辑和分析新生成的序列文件和DNA微卫星分型；和2。一种DNA数据存储系统，包括：用于随时检索和操作DNA序列文件的外部硬盘驱动器和用于长期、高稳定性存储DNA序列文件的磁光驱动器。Ruvolo博士及其同事的研究使用了DNA序列数据，购买这种仪器将成倍提高实验室的生产力。灵长类动物系统学和进化研究是实验室工作的重点，该团队目前参与了许多项目。这包括对灵长类动物分子系统学的广泛研究，以及对链霉素分子进化的补充研究。该小组已经开始确定现存黑猩猩的遗传多样性，并正在检查东非黑猩猩种群的线粒体DNA序列和核微卫星标记可变性。这些黑猩猩之所以重要，是因为它们处于濒危状态，而且有大量的行为信息可供选择。新的仪器还将允许研究小组扩大对类人猿核基因变异和基因组结构的研究。Ruvolo博士的实验室培养本科生和研究生以及博士后；自动测序设备将有助于这一点，以及研究，功能。

9414016 Ruvolo With National Science Foundation support, Dr. Maryellen Ruvolo will purchase an automated DNA sequencer system consisting of: 1. an Applied Biosystems automated DNA sequencer with the necessary software for editing and analyzing newly-generated sequence files and for DNA microsatellite typing; and 2. a DNA data storage system consisting of: an external hard disk drive for ready retrieval and manipulation of DNA sequence files and a magneto- optical drive for long-term, high stability storage of DNA sequence files. The research of Dr. Ruvolo and her colleagues uses DNA sequence data and the purchase of this instrumentation will exponentially increase the laboratory's productivity. Research on primate systematics and evolution constitutes the focus of the laboratory's work and the team is currently involved in a number of projects. These include a broad scale approach to the molecular systematics of primates and a complementary effort on the molecular evolution of the Strepsirhines. The group has begun work to determine the genetic diversity present within extant chimpanzees and is examining mitochondrial DNA sequence and nuclear microsatellite marker variability in East African chimpanzee populations. These chimpanzees are important because of their endangered status as well as the extensive behavioral information available on selected groups. The new instrumentation will also allow the team to expand its examination of hominoid nuclear gene variation and genomic structure. Dr. Ruvolo's laboratory trains undergraduate and graduate students as well as post-doctoral fellows; automated sequencer equipment will assist in this, as well as the research, function.