Acquisition of a Nuclear Magnetic Resonance Spectrometer

购买一台核磁共振光谱仪普林斯顿大学化学系向化学研究仪器和设施项目申请资金，用于购买一台核磁共振光谱仪。目前，普林斯顿大学唯一的高场核磁共振光谱仪是已经使用了7年的JEOL GSX-500。这个光谱仪是下列涉及化学教员及其小组的项目的关键限制因素。最近在溶液中蛋白质和大量水之间的分子间相干转移的演示为我们在生物分子核磁共振和成像中增强信号开辟了各种新的应用。采用高灵敏度探针和快速梯度脉冲（合作完成）的现代光谱仪上的600 MHz数据证明了该技术的重要性。现有光谱仪的500兆赫数据对这些研究基本上是无用的。多维核磁共振被广泛应用于一个旨在理解dna -碳水化合物相互作用的重大项目中。在这些系统中，500兆赫频谱显示出严重的频谱重叠，而在600兆赫频谱中，这种重叠已被证明大大减少。* Michael Hecht系统设计和合成新蛋白质的结构和性质的比较研究依赖于多核，多维高场核磁共振完成分配和溶液结构确定。在协作工作中已经开始了600兆赫的研究。*约翰T.格罗夫斯极高灵敏度的质子核磁共振是需要的小分子溶解在磷脂膜的结构调查，并为跨膜配体交换动力学的研究。目前的500兆赫对这项工作不够敏感，这项工作是研究细胞膜功能和动力学的广泛计划的一部分。* Jannette Carey蛋白质折叠和稳定性的研究，以及这些特性与配体结合活性的关系，需要使用现代600兆赫光谱仪提供的色散，获得多维核磁共振能力。这些研究领域，跨越生物结构和物理化学，提出了一套极其多样化的要求，只能满足600兆赫光谱仪。此外，增加第二个高场将提供必要的能力，以较少限制地利用现有的500兆赫光谱仪。

9414192 Purchase of a Nuclear Magnetic Resonance Spectrometer The Department of Chemistry at Princeton University requests funds from the Chemistry Research Instrumentation and Facilities program for the purchase of a nuclear magnetic resonance (NMR) spectrometer. Currently, the only high field NMR spectrometer at Princeton University is a seven year old JEOL GSX-500. This spectrometer is the critical limiting factor to the following projects involving Chemistry faculty members and their groups. * Warren S. Warren Recent demonstrations of intermolecular coherence transfer between proteins and bulk water in solution open us a variety of novel applications to enhance signals in biomolecular NMR and imaging. 600 MHz data on a modern spectrometer with high sensitivity probes and fast gradient pulses (done in collaboration) have proven the importance of the technique. 500 MHz data with the existing spectrometer are essentially useless for these studies. * Dan Kahne Multi-dimensional NMR is used extensively in a major project directed towards understanding DNA-carbohydrate interactions. 500 Mhz spectra show severe spectra overlap in these systems, which have been shown to be substantially reduced in 600 MHz spectra. * Michael Hecht Systematic design and synthesis of novel proteins for compartive studies of structure and properties depend on multi-nuclest, multi-dimensional high-field NMR for complete assignment and solution structure determination. 600 Mhz studies have been started in collaborative work. * John T. Groves Extremely high sensitivity proton NMR is required for structural investigations of small molecules dissolved in phospholipid membranes, and for studies of kinetics of transmembrane ligand exchange. The current 500 Mhz is not sufficiently sensitive for much of this work, which is part of a broad program to study the function and dynamics of cellular membranes. * Jannette Carey Studies of protein folding and s tabilty and the relation of these properties to ligand-binding activity require access to multi-dimensional NMR capabilties with the dispersion afforded by a modern 600 MHz spectrometer. These research areas, spanning biostructural and physical chemistry, present an extremely diverse set of requirements that can only be met by a 600 MHz spectrometer. Moreover, the addition of a second high field would provide necessary capacity for less restrictive utilization of the existing 500 MHz spectrometer.