Coupled Multiscale Solidification Processes in Multicellular Tissues

摘要 拟议的工作将解决多细胞组织冷冻过程中发生的热和质量传输现象。 将通过使用定向梯度凝固阶段和等温低温灌注显微镜阶段的凝固过程和组织传输特性进行实验研究。 将进行瞬态有限元分析，以研究全球热场和界面形态场的相互作用发生在一个长度尺度的几个数量级小于热场。 结合实验和计算研究在微观和宏观尺度的运输的独特方法有很好的潜力，以提高在多细胞组织冷冻的基本理解，并导致更好的处理协议的组织和冷冻手术的冷冻保存。

ABSTRACT The proposed work will address thermal and mass transport phenomena occurring during freezing of multicellular tissues. The solidification process and tissue transport properties will be studied experimentally by using both a directional gradient solidification stage and an isothermal cryogenic perfusion microscope stage. Transient finite-element analyses will be performed to study the interaction of the global thermal fields and the interface morphology fields take place at a length scale several orders of magnitude smaller than the thermal field. The unique approach of combining experimental and computational investigations at the microscale and macroscale transport has a good potential to improve the fundamental understanding of freezing in multicellular tissues and lead to better processing protocols for cryopreservation of tissues and cryosurgery.