Optimization and Purification of Virus-Like Particles Produced in a Baculovirus/Insect Cell System

宾利病毒样颗粒（VLP's）是不含任何功能性核酸、DNA或RNA的病毒外壳蛋白结构。因此，它们为人类和动物病毒性疾病提供了安全、有效、多价和低成本的疫苗。Bentley教授和Vikharia建议利用杆状病毒/昆虫细胞系统生成嵌合蛋白来研究这一相对较新的现象。他们建议对影响这些颗粒生产和性能的变量进行基础研究。需要回答的问题包括：对于IBDV和禽呼肠孤病毒这两种重要的家禽病毒来说，VLP的组装需要什么结构蛋白？在什么水平上组装VLP ？什么因素影响VLP的表达？与VLP分离和稳定性相关的问题：病毒蛋白基因将取代杆状病毒AcNPV外壳蛋白polyhedrin，杆状病毒在体外的复制和维持不需要这种蛋白。＊＊＊

9633009 Bentley Virus-like particles (VLP's) are viral coat protein structures devoid of any functional nucleic acids, DNA or RNA. As such, they offer the possibility of safe, effective, multivalent, and low cost vaccines for human and animal viral diseases. Professor Bentley and Vikharia propose to study this relatively new phenomena using the well characterized baculovirus/insect cell system to generate the chimeric proteins. They propose a basic study of the variables that influence the production and perform and of these particles. Among the questions to be answered are: what structural proteins are necessary and at what levels for the assembly of VLP's for two important poultry virus, IBDV and avian reovirus? what factors influence the expression of the VLP's? and what problems are associated with VLP separation and stability: The viral protein genes will be substituted for the baculovirus AcNPV coat protein, polyhedrin, which is not required for the replication and maintenance of the baculovirus in-vitro. ***