Enviromentally Benign Synthesis of Aromatic Chemicals from Carbohydrates

这项工作的重点有四个方面。首先，将制定方法 其中生物催化合成的肌醇氢化芳族化合物， 非生物转化为连苯三酚，间苯三酚， 羟基对苯二酚、对苯二酚、邻苯二酚和间苯二酚。 二是 D-葡萄糖在E.大肠杆菌的基因会被改变， D-葡萄糖转化为3-脱氢莽草酸的产率，3-脱氢莽草酸是制备D-葡萄糖的关键中间体。 生物催化合成己二酸、邻苯二酚和没食子酸。 第三、 将通过表达P产生对苯二酚的生物催化途径。 编码香草酸羟化酶的基因 对羟基苯甲酸酯合成E.杆菌 第四，生物催化 间苯二酚的合成将使用编码 没食子酸合成酶和没食子酸降解酶， K. pneumoniae、P. acidigallici和E.氧化还原。 与此 环境友好化学合成和加工奖， 合成有机计划是支持博士约翰W。霜 他是密歇根州立大学化学系的教授。 弗洛斯特医生 他将把工作重点放在开发合成方法上， 从碳水化合物经济地衍生的各种化学品 原料。 通过这种方式，无毒的D-葡萄糖、D-木糖和L-阿拉伯糖， 来源于可再生的玉米淀粉和纤维，可替代致癌物质 苯，来自不可再生的化石燃料，作为合成原料 材料

The focus of this work is fourfold. First, methodology will be developed whereby biocatalytically synthesized inositol hydroaromatics are abiotically converted into pyrogallol, phloroglucinol, hydroxyhydroquinone, hydroquinone, catechol and resorcinol. Second, the uptake of D-glucose in E. coli will be altered to increase the percent yield of D-glucose into 3-dehydroshikimic acid, a key intermediate in the biocatalytic synthesis of adipic acid, catechol and gallic acid. Third, a biocatalytic route to hydroquinone will be created by expressing the P. chrysosporium gene encoding vanillate hydroxylase in p-hydroxybenzoate-synthesizing E. coli. Fourth, a biocatalytic synthesis of resorsinol will be developed using genes encoding gallate-synthesizing and gallate-degrading enzymes from P. blakesleeanus, K. pneumoniae, P. acidigallici and E. oxidoreducens. With this Environmentally Benign Chemical Synthesis and Processing award, the Synthetic Organic Program is supporting the research of Dr. John W. Frost of the Department of Chemistry at Michigan State University. Dr. Frost will focus his work on developing synthetic methodology that allows a variety of chemicals to be economically derived from carbohydrate feedstocks. In this way, nontoxic D-glucose, D-xylose and L-arabinose, derived from renewable corn starch and fiber, can replace carcinogenic benzene, derived from nonrenewable fossil fuels, as a synthetic starting material.