Structural and Functional Analysis of Photosystem I: Molecular Biological and Physico-Chemical Studies

蓝藻光系统I反应中心是一种光驱动氧化还原酶，催化电子从还原的细胞色素c553（或质体青素）转移到氧化的铁氧还蛋白（或黄氧还蛋白）。这项研究计划的长期目标是了解这种复杂酶的生物起源，以及结构和机制细节，使其能够催化上述反应，量子产率接近1.0，热力学效率非常高（~45%）。利用先前资助期间获得的知识，将使用位点定向诱变，蛋白质过剩生产，生化分辨率和重构，化学救援和修饰以及动力学光学和EPR光谱的强大方法来生产和分析具有新性质的PS I反应中心。植物、藻类和蓝藻的光合作用是地球上生物能和氧气的主要来源。研究者的工作将增加光合作用的知识，并将提供可能用于作物光合作用基因操作的信息

9723469 Bryant The cyanobacterial Photosystem I reaction center is a light-driven oxidoreductase catalyzing the transfer of electrons from reduced cytochrome c553 (or plastocyanin) to oxidized ferredoxin (or flavodoxin). The long-term goal of this research program is to understand the biogenesis of this complex enzyme, as well as the structural and mechanistic details that allow it to catalyze the above mentioned reaction with a quantum yield approaching 1.0 and a very high thermodynamic efficiency (~45%). Taking advantage of knowledge gained during the prior funding period, powerful methods of site-directed mutagenesis, protein overproduction, biochemical resolution and reconstitution, chemical rescue and modification, and kinetic optical and EPR spectroscopies will be used to produce and analyze PS I reaction centers with novel properties. Photosynthesis in plants, algae and cyanobacteria is the major source of biological energy and oxygen on the earth. The investigator's work will increase the knowledge of photosynthesis and will provide information that can potentially be used for genetic manipulation of photosynthesis in crop plants