Regulation and Function of Membrane Lipid Hydrolysis in Lipid-Based Signaling of Plants

9808729 Wang将生物和非生物信号转导到细胞通常需要磷脂酶的功能。 磷脂酶D（PLD）的活化产生第二信使，并参与广泛的细胞过程，包括细胞生长、增殖、分化、繁殖和防御反应。 本项目的目标是了解PLD发挥其细胞效应的机制，并确定PLD激活的细胞靶点。 为了阐明PLDs的细胞活化，本研究将解决PLDs是否受磷酸化调节以及不同PLDs如何与多磷酸肌醇相互作用并受其刺激。 为了确定PLD激活的下游靶点，本项目将确定PLD缺陷转基因拟南芥中蛋白质磷酸化和活性氧的产生是否改变，以及PLD激活是否影响其他脂质信号传导过程。 此外，PLD在植物激素，脱落酸，响应中的作用将被研究。 该项目的完成将填补植物生长和发育中PLD和基于脂质的信号传导的当前知识的关键空白。 这项研究还可能确定新的方法，以提高种子发芽，减少植物收获后和田间损失。

9808729 Wang Transduction of biotic and abiotic cues to the cell often requires the function of phospholipases. Activation of Phospholipase D (PLD) generates second messengers and is involved in a broad range of cellular processes, including cell growth, proliferation, differentiation, reproduction, and defense responses. The goals of this project are to understand the mechanisms by which PLDs exert their cellular effects and to identify cellular targets of PLD activation. To elucidate the cellular activation of PLDs, this study will address if PLDs are regulated by phosphorylation and how different PLDs interact with and are stimulated by polyphosphoinositides. To identify the downstream targets of PLD activation, this project will determine if protein physphorylation and reactive oxygen generation are altered in PLD-deficient transgenic Arabidopsis and if PLD activation affects other lipid signaling processes. In addition, the role of PLD in the phytohormone, abscisic acid, response will be investigated. Completion of this project will fill critical gaps in the current knowledge of PLDs and lipid-based signaling in plant growth and development. This study may also identify novel ways to enhance seed germination and to decrease plant post-harvest and in-field losses.