Structure, Assembly, and Host Interactions of Helical Plant Viruses

许多丝状植物病毒的结构将用纤维衍射法进行详细的分子结构测定。将要研究的病毒包括两种烟草病毒，即齿舌环斑病毒(ORSV)和大麻花叶病毒(SHMV)，以及FuroVirus、PoexVirus和PotyVirus组的成员。利用烟草花叶病毒(TMV)的已知结构，通过分子置换确定烟草花叶病毒的结构。其他结构将使用P.I.S实验室开发的方法从头确定。所有的结构都将使用分子动力学方法进行精炼，就像P.I.S实验室所采用的那样，用于光纤衍射数据。待确定的结构将对了解病毒的分子特征具有重要意义，并将促进对病毒组装和拆解、病毒与宿主的相互作用和专一性、媒介传播以及结构与RNA病毒快速进化之间的关系的了解。一些丝状植物病毒的结构将通过纤维衍射方法确定。将要研究的病毒包括两种烟草病毒，即齿舌环斑病毒(ORSV)和大麻花叶病毒(SHMV)，以及FuroVirus、PoexVirus和PotyVirus组的成员。烟草病毒和痘病毒在生物技术中的重要性越来越大。马铃薯Y病毒，尤其是马铃薯Y病毒在农业上具有重要意义。

M9809879StubbsThe structures of a number of filamentous plant viruses will be determined in molecular detail, using fiber diffraction methods. The viruses to be studied include two tobamoviruses, odontoglossum ringspot virus (ORSV) and sunn-hemp mosaic virus (SHMV), and members of the furovirus, potexvirus, and potyvirus groups. Structures of the tobamoviruses will be determined by molecular replacement, using the known structure of tobacco mosaic virus (TMV). The other structures will be determined de novo, using methods developed in P.I.'s laboratory. All structures will be refined using molecular dynamics methods, as adapted in P.I.'s laboratory for use with fiber diffraction data. The structures to be determined will be important in understanding the molecular characteristics of viruses, and will advance understanding of viral assembly and disassembly, viral-host interactions and specificity, and vector transmission, as well as the relationship between structure and the rapid evolution of RNA viruses.The structures of a number of filamentous plant viruses will be determined using fiber diffraction methods. The viruses to be studied include two tobamoviruses, odontoglossum ringspot virus (ORSV) and sunn-hemp mosaic virus (SHMV), and members of the furovirus, potexvirus, and potyvirus groups. Tobamoviruses and potexviruses are of increasing importance in biotechnology. Potexviruses and especially potyviruses are of major agricultural importance.