High Field NMR Spectrometer

该合同将支持购买750 MHz多核高分辨率核磁共振系统，主要用于密歇根大学化学/生物化学核磁共振社区。该仪器由NSF和NIH通过NSF/NIH共享仪器计划共同资助。该仪器将用于实验，以确定蛋白质、核酸和蛋白质-核酸复合物在溶液中的高分辨率三维结构和动力学。与目前可用的仪器相比，新仪器的主要优点是灵敏度至少提高了两倍，使这些更困难的系统的结构研究触手可及。受益于此次收购的项目有：分子伴侣蛋白Hsc-70和DnaK的结构研究，基质溶素及其抑制剂和底物复合物的结构研究，大肠杆菌ars -操纵子表达蛋白的结构研究，胞基酰基转移酶的结构阐明，半胱氨酸蛋白酶抑制剂的设计，改进的DNA结合碳水化合物的合成，DNA三螺旋的动力学，抗DNA自身抗体抗原相互作用的阐明，以及GABA受体的可溶性和跨膜结构域的结构。

This award will support the purchase of a 750 MHz multi- nuclear high-resolution NMR system primarily for the use of the University of Michigan Chemistry/Biological Chemistry NMR community. This instrument is jointly funded by NSF and NIH through the NSF/NIH Shared Instrumentation Program. The instrument will be used for experiments leading to the determination of high-resolution three-dimensional structures and dynamics of proteins, nucleic acids, and protein-nucleic acid complexes in solution. The major virtue of the new instrument is an improvement of at least a factor of two in sensitivity as compared to currently accessible instruments, bringing the structural studies of these more difficult systems within reach. Projects benefiting from this acquisition are: structural studies of the molecular chaperone proteins Hsc-70 and DnaK, structural studies of Stromelysin and its complexes with inhibitors and substrate, structural studies of proteins expressed by the E. coli Ars-Operon, structural elucidation of Cytidylyltransferase, design of Cysteine Protease inhibitors, synthesis of improved DNA binding carbohydrates, dynamics of DNA triple helices, elucidation of anti-DNA autoantibody antigen interactions, and the structure of soluble and membrane-spanning domains of the GABA receptor.