"An Integrated Approach to Instruction in Electrochemistry and Liquid Chromatography: A LabVIEW-Based Pulsed Amperometric Detection System"

传统的分析化学课程通常会花费大量的时间来研究电化学方法和分析分离。虽然大多数学生在理解和应用分离技术的基本原理方面没有什么困难，但电化学的基本原理往往更加抽象。不幸的是，大多数传统的电化学实验室练习很少使学生的主题变得生动。本提案中概述的实验室项目旨在为学生提供一个动态的互动平台，用于电化学和色谱教学，重点是开发液相色谱电化学检测方案（LC-EC）。一个为期四周的实验室序列，强调发展的LC-EC方法测定碳水化合物将被设计为我们的高级仪器分析课程。（基于此方法在质量控制中的应用的为期一周的练习将被纳入我们的讲师级入门分析课程。该项目的核心是基于LabVIEW的数据采集接口。每个电化学实验将由LabVIEW程序控制，通常被称为虚拟仪器，它让学生完全控制所有电极电位和定时参数。在对循环伏安法的基本原理进行了初步研究之后，葡萄糖的伏安行为将被应用于流通系统中碳水化合物检测方案的开发。学生将被引导发现，为了获得可再现的响应，需要脉冲电位序列。将开发一个多步电位波形（E与t），包括表面氧化层的形成和溶解，使学生能够在分子水平上可视化电化学过程。然后将该波形应用于通过阴离子交换液体色谱分离的碳水化合物的定量测定。

Traditional courses in analytical chemistry typically devote a significant amount of time to the study of both electrochemical methods and analytical separations. While most students have little difficulty understanding and applying the basic principles of separation techniques, the fundamentals of electrochemistry tend to remain much more abstract. Unfortunately, most traditional electrochemistry laboratory exercises do little to make the subject come alive for the student. The laboratory project outlined in this proposal is designed to provide students with a dynamic interactive platform for instruction in electrochemistry and chromatography by focusing on the development of an electrochemical detection scheme for liquid chromatography (LC-EC). A four-week laboratory sequence emphasizing the development of an LC-EC method for carbohydrate determinations will be designed for our senior-level instrumental analysis course. (A one-week exercise based on applications of this method for quality control will be incorporated into our sophomore-level introductory analytical course.) At the heart of this project is a LabVIEW-based data acquisition interface. Each electrochemical experiment will be controlled by a LabVIEW program, commonly referred to as a virtual instrument, which gives students complete control of all electrode potential and timing parameters. After an initial focus on the fundamentals of cyclic voltammetry, the voltammetric behavior of glucose will be applied to the development of a detection scheme for carbohydrates in a flow-through system. Students will be led to discover that a pulsed potential sequence is needed in order to obtain a reproducible response. A multi-step potential waveform (E vs. t) will be developed to include both formation and dissolution of a surface oxide layer, allowing the student to visualize electrochemical processes at the molecular level. This waveform will then be applied to the quantitative determination of carbohydrates separated by anion-exchange li quid chromatography.