Interdepartmental (Biology, Anthropology, Chemistry) Genetic Analysis Facility

在美国国家科学基金会的支持下，纽约大学的托德·迪索特尔博士和他的合作者将购买一台ABI PRISM 377系统，该系统包括一个电泳和检测装置、一台Power Macintosh计算机以及用于DNA测序分析或PCR片段大小确定和量化的软件。该系统将通过使用多色荧光技术实现高吞吐量，通过在同一通道上共同加载多个PCR或测序反应的产物来实现多重电泳法。为了提高使用科学家群体的效率，将获得一个热循环仪(Perkin-Elmer Geneamp PCR System 9700)以及一个微型离心机(Eppendorf 5400系列)和微型浓缩器(Savant DNA SpeedVac)，并将其与ABI遗传分析仪一起安装。Power Macintosh计算机将允许对ABI基因分析仪上收集的数据进行离线分析，从而使控制ABI机器的计算机专门用于数据收集。这一仪器将极大地提高多名研究人员追求的六个研究项目的效率。这些研究包括：1.杆状线虫形态发生和形态进化的遗传和发育机制；2.线虫性腺器官发生的遗传学；3.灵长类分子进化和种群遗传学；4.鱼类洞穴适应的进化遗传学；分裂酵母中与Ras G蛋白和微管相互作用的未知蛋白质；以及6.HMG蛋白对DNA结构的识别。这台机器还将在研究生和本科生水平上使用，从而发挥有价值的教育和研究功能。

With National Science Foundation support, Dr. Todd Disotell and his collaborators at New York University will purchase an ABI PRISM 377 system which consists of an electrophoresis and detection unit, a Power Macintosh computer and software for DNA sequencing analysis or PCR fragment sizing and quantification. The system will allow high throughput through the use of multicolored fluorescence technology, allowing multiplex electrophoreses by co-loading the products of multiple PCR or sequencing reactions in the same lane. To increase the efficiency of the group of utilizing scientists, a thermocycler (Perkin-Elmer Geneamp PCR System 9700) along with a microcentrifuge (Eppendorf 5400 Series) and microconcentrator (Savant DNA SpeedVac) will be acquired and housed with the ABI genetic analyzer. A Power Macintosh computer will allow data collected on the ABI genetic analyzer to be analyzed off-line, allowing the computer controlling the ABI machine to be dedicated to data collection. This instrumentation will greatly improve the efficiency of six research projects, pursued by a number of investigators. These include the study of 1. the genetic and developmental mechanisms of morphogenesis and the evolution of form in rhabditid nematodes; 2. genetics of organogenesis in the gonad of C. elegans; 3.primate molecular evolution and population genetics; 4. evolutionary genetics of cave adaptations in fish; unidentified proteins in fission yeast that interact with Ras G-proteins and microtubules; and 6. recognition of DNA structure by HMG proteins. The machine will also be used at both graduate and undergraduate levels and thus serve a valuable educational as well as research function.