Designer Particles for The Biological Sciences

奖项摘要-9876691该奖项将支持通过光刻和金属沉积制造一种新型金属颗粒，并在生物研究的重要领域测试这些颗粒。 这些设计者颗粒将被生产和测试用于植物和哺乳动物细胞的生物射弹介导的遗传转化。 用设计者颗粒进行的特定生物学测试研究包括（1）金设计者颗粒与常规钨和金颗粒之间通过生物射弹的植物遗传转化效率的比较研究（2）通过使用磁性镊子向磁性设计颗粒施加振荡力或静态力，包埋在模型组织中，以及（3）通过常规金颗粒和金设计者颗粒之间的生物射弹对哺乳动物细胞转化效率的比较研究。 这些研究将包括DNA疫苗、绿色荧光蛋白-肌球蛋白融合蛋白、控制视网膜中神经元过程组装和分解的分子、突变离子通道的递送。

Award Abstract - 9876691This award will support the fabrication of a new kind of metal particle, by lithography and metal deposition, and the testing of these particles in important areas of biological research. These designer particles will be produced and tested for biolistics-mediated genetic transformation of plant and mammalian cells. The particular biological testing studies to be conducted with the designer particles include (1) a comparative study of plant genetic transformation efficiency by biolistics between the gold designer particles and conventional tungsten and gold particles (2) the use of cobalt designer particles for the study of the viscoelastic response of model tissues by the using magnetic tweezers to apply oscillating or static forces to magnetic designer particles embedded in the model tissue, and (3) a comparative study of mammalian cell transformation efficiency by biolistics between conventional gold particles and gold designer particles. These studies will include the delivery of DNA vaccines, green fluorescent protein-myosin fusion proteins, and molecules controlling assembly and disassembly of neuronal processes in the retina, mutant ion channels.