Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
批准号:
9905669
负责人:
Eleftherios Papoutsakis
金额:
$81.83万
依托单位:
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-04-01 至 2004-03-31
中文摘要
该项目的目的是利用现代分子生物学和基因技术研究溶剂型梭状芽胞杆菌的代谢工程。这项跨学科建议的主要目标是从基因上改变乙酰丁酸梭菌的代谢。该建议描述了四个活性领域:(1)通过假定的抑制子SolR对Sol基因的调节进行详细研究;(2)对SporOA和ORF(OrfY)基因产物的功能进行分子分析,这两个基因产物都可能在Sol基因的表达中发挥重要作用;(3)利用cDNA阵列分析基因表达的模式;以及(4)进一步开发反义RNA作为代谢工程工具。
英文摘要
The objective of the project is to investigate the metabolic engineering of solventogenic Clostridia using modern molecular biology and genetic techniques. The primary goal of this interdisciplinary proposal is to genetically alter the metabolism of Clostridium acetobutylicum. The proposal describes four areas of activity; (1) a detailed examination of the regulation of the sol locus genes through a putative repressor, SolR; (2) a molecular analysis of the function of sporOA and of ORF (orfY) gene products, both of which are likely to be important in the expression of the sol locus genes; (3) an analysis of patterns of gene expression using cDNA arrays; and (4) the further development of antisense RNA as a metabolic engineering tool.
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批准号:1804741
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项目类别:Standard Grant
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资助金额:$50.0万
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财政年份:2018
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Differentiation Engineering for Beneficial Alteration of Cellular Traits
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批准号:0853490
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资助金额:$45.0万
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财政年份:2009
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负责人:Eleftherios Papoutsakis
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依托单位:
Collaborative Research: Genomic Approaches to Metabolic Engineering of Solventogenic Clostridia
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批准号:0824629
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项目类别:Continuing Grant
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资助金额:$12.48万
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财政年份:2008
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负责人:Eleftherios Papoutsakis
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依托单位:
Multilibrary Strategies for Developing Complex Transgenic Microbial Phenotypes
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批准号:0756451
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项目类别:Standard Grant
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资助金额:$0.0万
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财政年份:2008
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负责人:Eleftherios Papoutsakis
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依托单位:
Collaborative Research: Genomic Approaches to Metabolic Engineering of Solventogenic Clostridia
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批准号:0418157
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项目类别:Continuing Grant
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资助金额:$79.98万
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财政年份:2004
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负责人:Eleftherios Papoutsakis
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依托单位:
BIochemical Engineering XIII (BioChE-03)
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批准号:0331063
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项目类别:Standard Grant
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资助金额:$2.0万
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财政年份:2003
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负责人:Eleftherios Papoutsakis
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依托单位:
Metabolic Engineering (ME): Genomic and Genetic Approaches to Solvent Tolerance in Anaerobes
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批准号:0331402
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项目类别:Continuing Grant
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资助金额:$60.75万
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财政年份:2003
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负责人:Eleftherios Papoutsakis
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依托单位:
ME: Metabolic Engineering of Solvent Tolerance in Anaerobic Bacteria
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批准号:9911231
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项目类别:Continuing Grant
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资助金额:$34.42万
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财政年份:2000
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负责人:Eleftherios Papoutsakis
-
依托单位:
Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
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批准号:9632217
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项目类别:Continuing Grant
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资助金额:$53.11万
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财政年份:1996
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负责人:Eleftherios Papoutsakis
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依托单位:
Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
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批准号:9210108
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项目类别:Continuing Grant
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资助金额:$68.79万
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财政年份:1992
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负责人:Eleftherios Papoutsakis
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依托单位:
Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
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批准号:9209627
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项目类别:Continuing Grant
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资助金额:$55.5万
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财政年份:1992
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负责人:Eleftherios Papoutsakis
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依托单位:
Understanding Injury of Suspended Animal Cells in Mixed Bioreactors Due to Bubble Breakup
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批准号:9022472
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项目类别:Continuing Grant
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资助金额:$19.06万
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财政年份:1991
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负责人:Eleftherios Papoutsakis
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依托单位:
Genetic Analysis and Metabolic Engineering of Anaerobic Butyric-Acid Clostridia
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批准号:8912209
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项目类别:Continuing Grant
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资助金额:$43.36万
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财政年份:1989
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负责人:Eleftherios Papoutsakis
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依托单位:
Genetic Analysis and Metabolic Engineering of Anaerobic Butyric-Acid Clostridia
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批准号:8912094
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项目类别:Continuing Grant
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资助金额:$25.94万
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财政年份:1989
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负责人:Eleftherios Papoutsakis
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依托单位:
Instrumentation for Research in Animal-Cell Culture and Metabolic Engineering
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批准号:8906388
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项目类别:Standard Grant
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资助金额:$4.9万
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财政年份:1989
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负责人:Eleftherios Papoutsakis
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依托单位:
Presidential Young Investigator Award: Studies in Biochemical Engineering
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批准号:8896100
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项目类别:Continuing Grant
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资助金额:$21.9万
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财政年份:1987
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负责人:Eleftherios Papoutsakis
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依托单位:
Recombinant-DNA Alterations, and Genetic and Enzymatic Characterization of Anaerobic Fermentation Process Importantto Biocatalysis by Butric-Acid Bacteria (Clostridia)
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批准号:8613077
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项目类别:Continuing Grant
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资助金额:$55.43万
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财政年份:1986
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负责人:Eleftherios Papoutsakis
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依托单位:
Enzymatic Mechanisms and Regulation of Solvent Production in Butanol/Acetone Fermentation
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批准号:8518959
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项目类别:Continuing Grant
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资助金额:$11.0万
-
财政年份:1986
-
负责人:Eleftherios Papoutsakis
-
依托单位:
国内基金
海外基金
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