Upgrading the Core Facility for DNA Sequencing at the University of New Hampshire

奖励摘要9977187资金将用于为UNH核心设施购买新的自动化DNA测序设备，该设备具有改进的功能和更高的通量。该设施每年处理超过17,000个样品，这些样品由大学5个院系的22个学院的实验室提交。主要项目包括鱼类基因组图谱、鱼类和浮游动物种群遗传学、脊椎动物激素cDNA测序等。其他研究集中在真核细胞的信号转导机制、人类白血病的无脊椎动物模型、转基因大型藻类的开发、珊瑚共生体的鉴定、海洋无脊椎动物系统发育的研究以及脊椎动物视觉色素基因的测序。基因结构、功能和进化的研究继续推动着我们对DNA测序的需求。近年来，新的基因组方法在生物学的各个领域变得越来越普遍，并且大大增加了我们设施对基因分型服务的需求。新的测序设备将增强能力，增加容量，降低成本，提高新罕布什尔大学核心设施测序服务的可靠性。

Award Abstract 9977187 Funds will be used to purchase new automated DNA sequencing equipment with improved capabilities and higher throughput for the UNH core facility. This facility processes more than 17,000 samples per year which are submitted by the labs of some 22 faculty from 5 academic departments in the University. Major projects using the equipment include genome mapping of fishes, population genetics of fishes and zooplankton, and cDNA sequencing of vertebrate hormones. Additional studies are focussed on mechanisms of signal transduction in eucaryotic cells, invertebrate models of human leukemia, development of transgenic macroalgae, identification of coral symbionts, studies of marine invertebrate phylogeny, and sequencing of vertebrate visual pigment genes. Studies of gene structure, function, and evolution continue to drive demand for DNA sequencing in our research. New genomic approaches are becoming increasingly prevalent in all areas of biology, and have greatly increased the need for genotyping services at our facility in recent years. The new sequencing equipment will enhance the capability, increase the capacity, reduce the cost, and improve the reliability of sequencing services in the core facility at the University of New Hampshire.