SGER: Pulsed Laser Readout of Large Molecular Libraries on an Optical Fiber Support

这项授予威斯康星大学密尔沃基分校彼得·盖辛格教授的小额探索性研究(SGER)奖得到了化学系分析和表面化学项目的支持。该项目针对分析化学的两个具体而重要的领域：为传感器阵列开发灵敏和快速的检测方案，以及确定组合库中的活性成分的识别战略。这项研究使用了一种新的方法，将光纤分割成用于并行合成方案的区域。将反应物和荧光团一起添加到每个纤维段中。当用激光脉冲激发时，检测到荧光团的短暂激发。通过脉冲和返回信号之间的时间差来计算荧光团的位置。每种化合物的位置都是从合成方案中得知的。这允许快速识别库的活动组件。组合化学库在合成新化学物质中正变得越来越重要。准确、快速地鉴定文库中的活性物种是优化利用文库的关键。该项目的重点是开发另一种制备和筛选文库的技术。这使得能够快速和经济地识别可能在药物等应用中有效的物种。该SGER奖授予威斯康星大学密尔沃基分校的彼得·盖辛格教授，该奖项由化学系的分析和表面化学项目支持。该项目针对分析化学的两个具体而重要的领域：为传感器阵列开发灵敏和快速的检测方案，以及确定组合库中的活性成分的识别战略。这项研究使用了一种新的方法，将光纤分割成用于并行合成方案的区域。将反应物和荧光团一起添加到每个纤维段中。当用激光脉冲激发时，检测到荧光团的短暂激发。通过脉冲和返回信号之间的时间差来计算荧光团的位置。每种化合物的位置都是从合成方案中得知的。这允许快速识别库的活动组件。组合化学库在合成新化学物质中正变得越来越重要。准确、快速地鉴定文库中的活性物种是优化利用文库的关键。该项目的重点是开发另一种制备和筛选文库的技术。这使得能够快速和经济地识别可能在药物等应用中有效的物种。

This Small Grants for Exploratory Research (SGER) award to Professor Peter Geissinger of the University of Wisconsin-Milwaukee is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The project targets two specific and important areas of analytical chemistry; development of a sensitive and rapid detection scheme for a sensor array and an identification strategy to determine the active components in a combinatorial library. The research uses a novel approach of segmenting an optical fiber into regions that are used in a parallel synthesis scheme. Reactants, along with a fluorophore are added to each fiber segment. Upon excitation with a laser pulse, the evanescent excitation of the fluorophore is detected. The position of the fluorophore is calculated by the time difference between the pulse and the return signal. The position of each compound is known from the synthetic scheme. This allows the active components of the library to be quickly identified. Combinatorial chemistry libraries are becoming increasingly important in synthesis of new chemicals. Accurate and rapid identification of the active species in the library is essential for the optimal use of the library. This project focuses on developing an alternative technique for preparing and screening libraries. This allows quick and economical identification of species that might be effective in applications such as pharmaceuticals. This SGER award to Professor Peter Geissinger of the University of Wisconsin-Milwaukee is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The project targets two specific and important areas of analytical chemistry; development of a sensitive and rapid detection scheme for a sensor array and an identification strategy to determine the active components in a combinatorial library. The research uses a novel approach of segmenting an optical fiber into regions that are used in a parallel synthesis scheme. Reactants, along with a fluorophore are added to each fiber segment. Upon excitation with a laser pulse, the evanescent excitation of the fluorophore is detected. The position of the fluorophore is calculated by the time difference between the pulse and the return signal. The position of each compound is known from the synthetic scheme. This allows the active components of the library to be quickly identified. Combinatorial chemistry libraries are becoming increasingly important in synthesis of new chemicals. Accurate and rapid identification of the active species in the library is essential for the optimal use of the library. This project focuses on developing an alternative technique for preparing and screening libraries. This allows quick and economical identification of species that might be effective in applications such as pharmaceuticals.