BAC Resources for Comparative Biology in Selected Angiosperms

为开花植物系统树的关键分支提供PatersonBAC文库，将极大地加快揭开被子植物2亿年进化史的进程。在Poales和Brassicales上的大量先前投资分别使它们成为单子叶和双子叶植物的重要成核点，从那里可以接触到植物多样性，以进一步研究基因组大小、基因库和功能以及植物形态的进化。我们将从精心挑选的被子植物基因组中建立5个文库，总计423,936个BAC(见下文)，以支持对发育、进化和比较生物学中明确描述的问题的新研究。其中，两个(Ananas，仙人掌)将是分类学上已知的第一个BAC文库，其中两个将是一个物种复合体(Gossypium)中重要基因组类型(A，F)的第一个BAC文库，它特别适合于提出关于形态转化、多倍体进化和基因组组织的基本问题。这些文库将分别在单子叶植物和双子叶植物中进行新的研究，通过与许多已经研究得很好的分类群进行重要的新比较，分别研究与植物多样化有关的一般机制，以及可能解释主要形态发育转化的具体事件。这些文库还将为揭示多倍体的基因组后果提供新的机会，并为在分子水平上没有充分研究的其他重要科学特征的发育遗传学提供新的见解。我们将研究的所有分类群都与具有重要经济意义的作物密切相关，这些作物表现出通过人类选择而‘放大’的特征，因此它们本身就包括植物生长和发育的各个方面的植物学模型。这项工作将使许多科学协同效应通过与先前由拟南芥和水稻基因组计划、NSF植物基因组计划(在Poales和Malvales)、USDA-IFAFS(在Brassicales)和USDA-NRI基因组计划(Malvales)的投资相联系来实现。社区对与这一项目有关的决策的参与程度很高。我们的研究与强大的培训和推广计划密切相关，成功地吸引了本科生和在科学中代表性不足的群体。BACS基因组二项通用名称家族1C值(MBP)#覆盖菠萝科531 55,296 10xGossypium herbaceum A基因组棉Malvaceae 1785 92,160 5xGossypium Longicalyx F基因组棉Malvaceae 1592 92,160 6x Gossypioides kirkii Malvaceae 579 73,728 12x仙人掌仙人掌869 110,51092 x总数423,936

0208311PatersonBAC libraries for key branches of the flowering plant family tree will greatly accelerate progress in unraveling the 200-million year history of angiosperm evolution. Extensive prior investments in the Poales and Brassicales make them important nucleation points for the monocots and dicots, respectively, from which to reach out across plant diversity to further investigate evolution of genome size, gene repertoire and function, and plant morphology. We will make 5 libraries totaling 423,936 BACs from carefully-selected angiosperm genomes (see below) to empower new investigations of well-delineated questions in developmental, evolutionary, and comparative biology. Among these, two (Ananas, Opuntia) will be the first BAC libraries known in their taxonomic orders, and two will be the first for important genome types (A, F) within a species complex (Gossypium) that is especially facile for asking fundamental questions about morphological transformations, polyploid evolution, and genome organizationThese libraries will empower new investigations in monocots and dicots respectively, of general mechanisms associated with plant diversification, and the specific events that may account for major morphological-developmental transformations, by virtue of making possible important new comparisons to many taxa already well studied. These libraries will also offer opportunities to shed new light on the genomic consequences of polyploidy, and new insights into the developmental genetics of other scientifically important features that are inadequately studied at the molecular level. All of the taxa we will study are closely-related to economically-significant crops that exhibit features 'amplified' by human selection, and as such comprise botanical models in their own right for various aspects of plant growth and development.This work will empower many scientific synergies to be realized by links with prior investments by the Arabidopsis and Rice Genome Initiatives, NSF Plant Genome Program (in the Poales and Malvales), USDA-IFAFS (in the Brassicales), and USDA-NRI genome programs (Malvales). Community involvement in decisions relating to this project has been substantial. Our research is closely-tied to strong training and outreach programs with a successful history of engaging undergraduates and groups under-represented in the sciences.Binomial Common name Family 1C value (Mbp) # of BACs Genome coverage Ananas comosus pineapple Bromeliaceae 531 55,296 10xGossypium herbaceum A-genome cotton Malvaceae 1785 92,160 5xGossypium longicalyx F-genome cotton Malvaceae 1592 92,160 6xGossypioides kirkii Malvaceae 579 73,728 12xOpuntia cochellinifera prickly-pear cactus Cactaceae 869 110,592 10x TOTAL 423,936