Arabidopsis 2010: Array-Based Genotyping: An Affordable Alternative

将开发一种基于阵列的基因分型方法，作为拟南芥研究人员的工具。目前在F2群体中定位突变和创建重组近交系群体遗传图谱的限速步骤是，需要使用数百个遗传标记对大量单株进行基因分型。基于阵列的基因分型允许同时评估许多分子标记，这表明该方法是一种快速且经济有效的基因分型方法。将生产斑点寡核苷酸玻片微阵列，可用于检测哥伦比亚和Landsberg erecta拟南芥材料之间的大约500个插入/缺失差异(Indel)。一旦它们被开发出来，打印的寡核苷酸阵列将以成本价提供给拟南芥研究界。信息性寡核苷酸的DNA序列、用于测试阵列的重组自交系的标记数据以及用于订购印刷阵列的信息将在项目网站(http://bti.cornell.edu/jander/mapping_array).上公布公开可用的基因分型阵列将通过促进基于突变表型的基因快速鉴定来促进拟南芥2010计划的目标。学生教育是这个项目的一个重要方面，定义良好的阵列实验将为本科生提供极好的研究机会。此外，这种新的基因分型方法可以应用于其他具有过于复杂的基因组的生物，而不能使用目前可用的基于阵列的方法进行有效的基因分型。

An array-based genotyping method will be developed as a tool for Arabidopsis researchers. The current rate-limiting step or mapping mutations in F2 populations and for creating genetic maps of recombinant inbred line populations is that large numbers of individual plants need to be genotyped using several hundred genetic markers. Array-based genotyping permits the simultaneous assessment of many molecular markers, suggesting this approach as a rapid and cost-effective genotyping method. Spotted oligonucleotide glass slide microarrays that can be used to detect approximately 500 insertion/deletion differences (InDel) between the Columbia and Landsberg erecta Arabidopsis accessions will be produced. Once they have been developed, printed oligonucleotide arrays will be made available to the Arabidopsis research community at cost. The DNA sequences of the informative oligonucleotides, marker data for recombinant inbred lines used to test the arrays, and information for ordering printed arrays will be published on the project web site (http://bti.cornell.edu/jander/mapping_array). Publicly available genotyping arrays will promote the goals of the Arabidopsis 2010 program by facilitating the rapid identification of genes based on mutant phenotypes. Student education is an important aspect of this project, and well-defined array experiments will provide excellent undergraduate research opportunities. In addition, this novel genotyping approach can be applied to other organisms with genomes that are too complex for efficient genotyping using currently available array-based methods.