High-density Genetic Map of Maize Transcripts

为了促进科学进步和作物改良，在玉米基因组测序项目中发现的数千个玉米基因必须相对于遗传图谱进行排序。该项目建立在美国国家科学基金会之前对玉米基因组学的投资基础上，以绘制4500个基因的遗传图谱。由此得到的高密度转录图谱将增强我们对玉米基因组的组织和进化的理解。这些数以千计的定位基因还将提供必要的基于序列的交叉链接，以促进水稻物理图谱与玉米遗传图谱的比对。这一资源将为确定玉米基因在玉米染色体上的线性顺序提供一种手段。还将产生1000个适用于各种遗传学实验的共显性遗传标记(插入缺失多态S)并分发给公众。研究计划在分子基因组学和计算基因组学之间为不同的本科生和研究生群体提供大量跨学科的培训机会。该项目将使用各种机制，让来自研究型大学、本科学院和服务不足的机构的本科生参与进来。这些学员将获得密集的指导性研究经验。为了帮助他们在全球环境中为科学生涯做准备，本科生将参观位于墨西哥的国际玉米和小麦改良中心(西班牙语首字母缩写CIMMYT)。受训者还将被鼓励回到他们毕业的高中，就他们的研究活动做简短的报告。其他外展活动将包括与爱荷华州立大学莱曼花园(http://www.reimangardens.iastate.edu))合作开展的一项计划，让小学生接触植物生物学。交付成果：将生成4,500个绘制的基因图谱和1,000个共显性遗传标记(插入删除多态[S])，并通过项目数据库(http://pslab.agron.iastate.edu/research/genomics/htp_est/idps.shtml)以及公共数据库(如PlantGDB(http://www.zmdb.iastate.edu/PlantGDB/)，))向公众分发MaizeGDB(http://www.maizegdb.org/)，和Gramene(http://www.gramene.org/).

To facilitate scientific advances and crop improvement, the thousands of maize genes being discovered during the maize genome sequencing project must be ordered relative to a genetic map. This project builds on prior NSF investments in maize genomics to genetically map 4,500 genes. The resulting high-density transcript map will enhance our understanding of the organization and evolution of the maize genome. These thousands of mapped genes will also provide the necessary sequence-based cross-links to facilitate the alignment of the rice physical map with the maize genetic map. This resource will provide a means to determine the linear order on maize chromosomes of maize genes. One thousand co-dominant genetic markers (Insertion Deletion Polymorphisms [IDP]s) suitable for use in a wide variety of genetic experiments will also be generated and distributed to the public.The research plan provides numerous, interdisciplinary training opportunities for a diverse group of undergraduate and graduate students at the interface between molecular and computational genomics. The project will use a variety of mechanisms to involve undergraduate students from a research university, an undergraduate college and underserved institutions. These trainees will be provided with intensive mentored research experiences. To help prepare them for scientific careers in a global environment, undergraduate students will visit to the International Maize and Wheat Improvement Center (Spanish acronym CIMMYT) in Mexico. Trainees will also be encouraged to return to the high schools from which they graduated to make short presentations regarding their research activities. Other outreach activities will include a program conducted in partnership with the Reiman Gardens at Iowa State University (http://www.reimangardens.iastate.edu) to expose elementary school students to plant biology.Deliverables:4,500 mapped genes and 1000 co-dominant genetic markers (Insertion Deletion Polymorphisms [IDP]s) will be generated and distributed to the public via the project database (http://pslab.agron.iastate.edu/research/genomics/htp_est/idps.shtml) as well as public databases such as PlantGDB (http://www.zmdb.iastate.edu/PlantGDB/), MaizeGDB (http://www.maizegdb.org/), and Gramene (http://www.gramene.org/).