SENSORS: Multi-Analyte Affinity Micro-Columns with Amplified Multi-Parameter Fluorescence Detection

来自工程学院、艺术与科学学院和新墨西哥大学健康科学中心的研究人员共同参与了这一合作项目，以解决开发一种新的、多功能和自适应的多分析物传感平台中的关键问题。这一合作研究计划将与其他大学的研究人员、国家实验室的研究人员和当地产业界密切联系。它将开发包括传感化学、仿生纳米材料、微流体和新型光学换能器在内的广泛技术，以产生具有可靠高性能特征的集成传感器系统。该项目代表着基础研究和应用研究的协同结合，这是开发基于亲和微柱的新一代高性能生物传感器系统所必需的。传感器阵列系统将允许同时、快速、可靠地测定各种痕量分析物。模型分析物被选择来代表重要的化学和生物物种的广泛类别。它们还与当前在痕迹检测方面的重大挑战直接相关，最显著的是在化学和生物战防御以及疾病早期检测方面。关键技术开发分为四个不同的工作领域，将开发：(1)用于微珠上放大分子转导的生物分子组件，(2)样品引入和通过多分析物亲和微柱泵送的最佳方法，(3)能够可靠地检测亲和微柱中反应的多参数荧光仪器，以及(4)系统集成、优化和测试。技术方法利用了一个跨学科团队的广泛专业知识，该团队最近在生物传感器技术和系统的开发方面进行了富有成效的合作。通过与业界的合作，还提出了一种明确的机制，用于将传感器系统与最先进的空气采样技术进行集成。该研究计划将通过IGERT、REU和RET计划以及全州范围内的纳米技术倡议，为来自代表性不足群体的学生提供重要的参与机会。

Researchers from the School of Engineering, College of Arts and Sciences and the Health Science Center at the University of New Mexico have teamed in this collaborative project to address critical issues in the development of a new, versatile and adaptive multi-analyte sensing platform. This collaborative research initiative will closely interface with researchers at other Universities, at the National Laboratories and with local industry. It will develop a wide range of technologies including sensing chemistry, biomimetic nanomaterials, microfluidics, and novel optical transducers to yield an integrated sensor system characterized by reliable high performance.This project represents a synergistic combination of fundamental and applied research that is requisite for the development of a new generation of high performance biosensor systems based upon affinity microcolumns. The sensor array systems will allow simultaneous, fast, reliable determination of a wide range of trace analyte species. Model analytes are chosen to be representative of broad classes of important chemical and biological species. They are also directly relevant to current grand challenges in trace detection, most notably in chemical and biological warfare defense and in early detection of disease. Critical technology development is grouped into four distinct work areas that will develop: (1) biomolecular assemblies for amplified molecular transduction on microbeads, (2) optimum methods for sample introduction and pumping through multi-analyte affinity microcolumns, (3) multiparameter fluorescence instrumentation capable of reliable detection of reactions in affinity microcolumns, and (4) system integration, optimization and testing. The technical approaches leverage the broad range of expertise of an interdisciplinary team with a recent history of productive collaboration in the development of biosensor technology and systems. A clear mechanism for integration of the sensor systems proposed with state-of-the-art air sampling technology is also presented, through collaboration with industry. The research program will offer significant opportunities for participation of students from underrepresented groups through IGERT, REU, and RET programs and a statewide initiative in nanotechnology.