SGER: Dynamics, Identification and Control of an Optical Tweezer System

提出了开展光镊动力学建模、辨识和反馈控制的实验和理论研究计划。通过适当地使用反馈控制和识别技术，我们计划将光镊作为传感器的精度和带宽提高几个数量级。这些技术的试验平台应用是使用镊子实时感知生物分子的机械或化学变化，例如DNA的机械“解链”。目前这种感知技术受到热噪声（朗之万力）和分子本身内部动力学的影响。技术提出，简化分子的动力学模型以及递归系统identificationalgorithms估计参数的陷阱分子interactionin存在显着的背景噪声。拟议的研究计划将包括开发适当的模型formolecule-trap相互作用，反馈控制和识别，并在现有的和operational光镊仪器在加州大学旧金山分校的PI实验室的算法进行实验测试。

It is proposed to carry out an experimental and theoreticalprogram of research related to the dynamical modelling,identification and feedback control of optical tweezers. By theproper use of feedback control and identification techniques, we plan to increase the accuracy and bandwidth of the optical tweezer as a sensor by orders of magnitude. The testbed applicationfor these techniques is to use the tweezer to sense, inreal time, mechanical or chemical changes to a biologicalmolecule; for example, the mechanical ``unzipping'' of DNA.Current techniques for such sensing are limited by the effects ofthermal noise (Langevin forces) and the internal dynamics ofthe molecule itself. Techniques are proposed that incorporatedynamical models of the molecule as well as recursive system identificationalgorithms to estimate the parameters of trap-molecule interactionin the presence of significant background noise. The proposedresearch program will consist of developing appropriate models formolecule-trap interaction, feedback control and identification,and experimental testing of the algorithms on the existing andoperational optical tweezer instrument in the PIs laboratory atUCSB.