Sensors: On-sensor Self-Calibration of Oxygen Microsensor; A Model System of Structural and Functional Integration of Biomicrosystem

标题：传感器：微溶氧传感器的传感器自校准：生物微系统结构与功能集成的模型系统摘要本工作的目的是利用集成的电化学驱动微系统实现溶氧传感器的片上、内建智能控制（2点自校准/自诊断）。智能化、微型化生化传感器的发展还处于起步阶段。需要开发一种新的方案来克服基线漂移和灵敏度下降引起的信号不稳定性。我们建议使用电化学驱动机制（水电解）来实现这一新功能。水电解微系统（微室，微电极，和微通道）将被设计为提供两个关键功能。首先，电化学产生的溶解气体饱和的电解质作为集成微传感器的校准物（含氧饱和和贫氧电解质的两点过程）;其次，电化学产生的气泡为样品溶液的取样/分配提供双向微流体操纵的驱动力。采用微细加工技术制备了一种由电流型溶解氧微传感器和水电解微驱动器组成的微系统。它们的性能将根据驱动信号来表征自校准/自诊断能力预计将显著提高生化传感器在连续使用期间的准确性和可靠性，并做出原位决策。使过程在生物和医学监测中可行。建议活动的智力价值电化学驱动是生物医学领域的新兴领域。基于MEMS的微传感器技术。该方法是第一种实现生物化学传感器/执行器功能集成（内置智能）与结构集成（小型化）相结合的方法。电解驱动可用于提高其他利用氧化酶反应的酶微传感器的性能，实现智能光学生物传感系统。同样的方法可以实现微流体驱动，而不需要复杂的MEMS结构。这两个微传感器和微执行器的系统集成预计将推进当前的biomicrosystem技术对innovativeinstrumentation自主和最少参加生化monitoring.broaderimplementing从拟议的activityThe PI有一个共同任命的部门电气计算机工程和生物科学在密苏里大学罗拉（UMR）.& PI的主要职责是通过发起全校范围的跨学科研究活动和教育来自两个部门的学生来弥合学科之间的差距。UMR是LS-MoAMP（路易斯斯托克斯密苏里州少数民族参与联盟）计划的成员，并运行威斯（妇女在工程和科学计划）。拟议中的研究将是一个宝贵的资源，通过来自各学术部门和代表性不足的群体的学生的参与，加快这些活动。

TITLE: Sensors: On-sensor self-calibration of oxygen microsensor; a model system of structuraland functional integration of biomicrosystem.ABSTRACTThe objective of this work is the implementation of on-chip, built-in intelligent control (2-pointself-calibration/self-diagnosis) of dissolved oxygen microsensor by using an integratedelectrochemical actuation microsystem. The development of intelligent and autonomousbiochemical microsensor is in infant stage. A novel protocol needs to be developed to overcomethe signal instability caused by drift of baseline and degradation of sensitivity. We propose touse an electrochemical actuation mechanism (water electrolysis) to accomplish this novelfunctionality. A water electrolysis microsystem (microchambers, microelectrodes, andmicrochannels) will be designed to provide two critical functions. First, the electrolytes saturatedwith the electrochemically generated dissolved gases serve as the calibrants for an integratedmicrosensor (two-point procedure with oxygen-saturated and oxygen-depleted electrolytes).Secondly, the electrochemically generated bubbles provide a driving force of the bidirectionalmicrofluidic manipulation for the sampling/dispensing of sample solution. A microsystemincluding an amperometric dissolved oxygen microsensor and a water electrolysis microactuatoris prepared by microfabrication technology. Their performances will be characterized in terms ofthe actuation signal (duration and amplitude) and the geometries of microfluidic components.The self-calibration/self-diagnosis capability is expected to significantly improve the accuracyand reliability of the biochemical sensors during continuous use and to make the in situ decision-making process viable in biological and medical monitoring.INTELLECTUAL MERITS OF PROPOSED ACTIVITYThe electrochemical actuation is an emerging field in the MEMS-based microsensortechnologies. The proposed method is the first approach to accomplish the functional integration(built-in intelligence) combined with the structural integration of biochemical sensor/actuator(miniaturization). The electrolysis actuation is applicable to improve performances of otherenzymatic microsensors that utilize the oxidase enzyme reaction and to accomplish intelligentoptical biosensing system. The same method can achieve the microfluidic actuation without thecomplicated MEMS-based structures. This system integration of both microsensors andmicroactuators is anticipated to advance current biomicrosystem technology towards innovativeinstrumentation for autonomous and minimally-attended biochemical monitoring.BROADER IMPACTS RESULTING FROM THE PROPOSED ACTIVITYThe PI has a joint appointment in the Departments of Electrical & Computer Engineering andBiological Sciences at the University of Missouri-Rolla (UMR). The primary responsibility ofthe PI is to bridge the gap between the disciplines by initiating the campus-wide interdisciplinaryresearch activities and by educating the students from both departments. The UMR is a memberof LS-MoAMP (The Louis Stokes Missouri Alliance for Minority Participation) program andruns WIES (Women in Engineering and Science Program). The proposed research will be avaluable resource to expedite these activities through the involvement of students from variousacademic departments and from underrepresented groups.