Chlamydomonas Resource Center

项目摘要 该奖项再次支持收集2300株单细胞绿色真菌，由C. reinhardtii和密切相关的物种。 披衣细胞是研究光合作用、鞭毛生物发生和功能、信号转导以及细胞生物学其他方面的极好模型系统。 最近完成的衣原体基因组序列可能会加速采用这种生物体作为实验工具。 这个收藏品，现在被称为衣原体资源中心（以前是杜克大学的衣原体遗传学中心），作为接收、编目、保存和分发野生型和突变型C。reinhardtii和其他衣原体物种，其中已进行了广泛的遗传分析。 它也是向国际科学界传播关于这种生物的信息的资源中心。 由于其易于培养和引人注目的鞭毛运动，衣原体也是高中生和大学生实验室练习的理想生物体。 因此，该中心还为有兴趣在课堂和个人科学项目中采用衣原体的学生和教师提供材料和建议。 除了活培养物，该中心还维护和分发用于衣原体研究的分子试剂，包括衣原体核、叶绿体和线粒体基因的基因组和cDNA克隆，以及cDNA文库。 一个网站[http://www.chlamy.org]提供了所有文化的收藏，历史信息和遗传基因座和突变等位基因的参考资料，遗传和分子图谱，地址目录和广泛的书目的描述。

Project Summary This award renews support for a collection of 2300 strains of the unicellular green alga consisting of mutants of C. reinhardtii and closely related species. Chlamydomonas is an excellent model system for studies of photosynthesis, flagellar biogenesis and function, signal transduction, and other aspects of cell biology. The recent completion of the genome sequence of Chlamydomonas will likely accelerate the adoption of this organism as an experimental tool. The collection, now called the Chlamydomonas Resource Center (formerly, the Chlamydomonas Genetics Center at Duke University), serves as the central repository to receive, catalogue, preserve and distribute wild type and mutant cultures of C. reinhardtii and other Chlamydomonas species in which extensive genetic analysis has been done. It also serves as the resource center to disseminate information on this organism to the international scientific community. Given its ease of culture and striking flagellar motility, Chlamydomonas is also an ideal organism for laboratory exercises for high school students and college undergraduates. Thus, the Center also provides materials and advice for students and teachers interested in adopting Chlamydomonas for classroom and individual science projects. In addition to live cultures, the Center also maintains and distributes molecular reagents useful for research on Chlamydomonas, including genomic and cDNA clones of Chlamydomonas nuclear, chloroplast and mitochondrial genes, and cDNA libraries. A web site [http://www.chlamy.org] provides descriptions of all cultures in the collection, historical information and reference material on genetic loci and mutant alleles, genetic and molecular maps, an address directory, and an extensive bibliography.