A Soft Polymer-on-Silicon Nano Photonic Device for High-Speed Fluorescence Multi-Spectrum Acquisition in Integrated Microfluidic Immunoassay System

本研究的目的是开发一种新型的纳米光子可调谐器件，用于高速单探测器光谱测量。 所提出的装置被集成在一个微流体系统中，以实现高通量的多分析物检测的流通微球为基础的荧光免疫分析，具有简单的光学和较少的计算要求。该方法是基于新的聚合物-硅混合微机电系统技术。它允许在三维聚合物微结构的表面上压印纳米级特征。该系统的光谱采集速度预计将超过100 nm/ms，并允许在微流体系统中的实时光谱。知识价值：本研究开发的创新纳米光子技术将指导未来波长鉴别检测的进步，用于识别和定量多种化学和生物物种。与微流控细胞培养和免疫测定系统相结合，该装置导致了一种不存在的科学仪器的发展，该仪器允许在微流控通道中原位监测细胞参数的时间变化。更广泛的影响：开发的微流控荧光免疫分析系统可能会发现新的商业市场，因为它的成本效益和生命科学研究和开发的实用性。 拟议的项目将促进培养新一代工程师和科学家的绝佳机会，他们将跨越传统研究领域的界限，创造新的研究途径。

0601237KurabayashiThe objective of this research is to develop a novel nano photonic tunable device for high-speed single-detector spectral measurement. The proposed device is integrated in a microfluidic system to achieve high-throughput multi-analyte detection in flow-through microsphere-based fluoroimmunoassay with simple optics and with less computational requirements. The approach is based on the new polymer-silicon hybrid microelectromechanical systems technology. It allows imprinting of nanoscale features on the surface of the three-dimensional polymer microstructure. The spectrum acquisition speed of the system is expected to exceed 100 nm/ms and to allow real-time spectroscopy in a microfluidic system. Intellectual merit:The innovative nano photonic technology developed in this research will guide future advancements of wavelength-discriminating detection for the identification and quantification of multiple chemical and biological species. Integrated with microfluidic cell culture and immunoassay systems, the device leads to development of a non-existing scientific instrument that permits in-situ monitoring of time variations of cellular parameters in a microfluidic channel. Broader Impact:The developed microfluidic fluoroimmunoassay system may find new commercial markets because of its cost-effectiveness and utility in life sciences research and development. The proposed project will promote an excellent opportunity to train a new generation of engineers and scientists who will cross the boundaries of traditional research fields and create new avenues of research.