Cellular localization and signal transduction of a novel family of olfactory receptors

嗅觉感受器被气味激活是神经元处理嗅觉刺激的第一步。随后的信号转导级联是不同的嗅觉受体基因的不同家庭的特点，并决定了相应的嗅觉受体神经元的处理特性。我们已经确定了一个新的嗅觉受体家族，即奥拉斯，它们是GPCR，与哺乳动物VIR信息素受体基因家族相关。ORA家族具有几个新的和意想不到的特性，因此是高度interests.With此应用程序，我们希望确定，这三种已知类型的嗅觉受体神经元表达的ora基因。利用共定位研究，我们将发现，哪些G蛋白和离子通道可能有助于ORA受体的信号转导。竞争和剥夺实验以及配体对受体的激活将证明候选信号转导分子的贡献。我们将在斑马鱼中对表达奥拉的受体神经元进行基因标记，以确定它们的靶肾小球。

The activation of olfactory receptors by odors constitutes the first step in the neuronal processing of olfactory stimuli. The subsequent signal transduction cascade is characteristically different for the different families of olfactory receptor genes and determines the processing properties of the corresponding olfactory receptor neurons. We have identified a novel family of olfactory receptors, the ORAs, which are GPCRs and related to the mammalian VIR pheromone receptor gene family. The ORA family exhibits several novel and unexpected properties and is therefore of high interest.With this application we wish to determine, which of the three known types of olfactory receptor neurons expresses the ora genes. Using co-localization studies we will find out, which G proteins and ion channels may contribute to signal transduction of ORA receptors. Competition and deprivation experiments together with activation of receptors by ligands will prove the contribution of candidate signal transduction molecules. We will genetically label the ORA-expressing receptor neurons in zebrafish to determine their target glomeruli.