EFRI-CBE Topic B: Biofunctionalized Devices - On Chip Signaling and "Rewiring" Bacterial Cell-Cell Communication

PI姓名：W.E. Bentley机构：马里兰州大学生物技术研究所提案编号：0735987 EFRI-CBE：生物功能化芯片上的信号传导和重新布线细菌细胞-细胞通信摘要本项目是通过使用基于器件的电信号来指导微机电系统（MEMS）内的生物合成途径、基于细胞的传感器和基于细胞的致动器的组装来展示信号翻译，并使用板上的电、磁、电、电、磁、电、磁、电、机械和光学系统，以反馈和引导基于细胞的系统朝向用户指定的结果。该项目的目标是在称为群体感应的过程中由细菌信号自诱导物介导的细胞间通讯系统。主要研究者（PI）已经创建了一个计算模型，该模型捕获了群体信号产生、受体驱动识别和摄取的动态。这个模型，基于生物化学和生物物理过程，将指导概念设计的子系统的candions组装架构，指导异源蛋白质合成响应特定的生物分子线索。将向细胞发出信号以启动生物膜形成和成熟。MEMS环境将首次为这种基于细胞的信号转导过程的设计、构建和测试提供实验平台。此外，这种MEMS环境将检测细胞功能，并通过引导信号通路，以受控和定向的方式改变细胞表型。MEMS学生将在鼓励多样性和促进合作的多学科环境中追求基本问题和技术目标。学生将学习从分子和细胞生物学到信号处理的科学，并接触到设备制造和使用的问题。PI将寻求行业的指导和支持。该研究将在器件制作、嵌入式传感器系统、细菌致病性、生物膜形成、基因调控和信号转导等方面产生新的成果。展望了影响医学（药物发现，合成和递送），通信（生物功能化微制造设备）和安全（智能传感器）领域的发展。

PI name: W.E. BentleyInstitution: University of Maryland Biotechnology InstituteProposal Number: 0735987EFRI-CBE: Biofunctionalized Devices On Chip Signaling and Rewiring Bacterial Cell-Cell CommunicationAbstractThis project is to demonstrate signal translation by employing device-based electrical signals to guide the assembly of biosynthetic pathways, cell-based sensors, and cell-based actuators within a microelectromechnical system (MEMS), and to use on-board electrical, magnetic, mechanical, and optical systems to feedback and guide the cell-based system towards user-specified outcomes. The target of this project is the cell-cell communication system mediated by bacterial signaling autoinducers in a process known as quorum sensing. The Principal Investigators (PIs) have created a computational model that captures the dynamics of quorum signal generation, receptor driven recognition, and uptake. This model, based on biochemical and biophysical processes, will guide the conceptual design of subsystem synthons assembled architectures that guide heterologous protein synthesis in response to specific biomolecular cues. Cells will be signaled to initiate biofilm formation and maturation. The MEMS environment will enable for the first time, an experimental platform for the design, construction, and testing of this cell-based signal transduction process. Moreover, this MEMS environment will detect cell function and, by guiding signaling pathways, change cell phenotype in a controlled and directed manner.Engaged students will pursue both fundamental questions and technological goals within a multi-disciplinary environment that encourages diversity and fosters cooperation. Students will learn sciences that range from molecular and cell biology to signal processing and be exposed to issues of device fabrication and use. The PIs will enlist guidance and support from industry. This research may spawn new efforts on device fabrication, embedded sensor systems, bacterial pathogenicity, biofilm formation, genetic regulation and signal transduction. Developments are envisioned that impact fields of medicine (drug discovery, synthesis, and delivery), communications (biofunctionalized microfabricated devices), and security (smart sensors).