Collaborative Research: Biomagnetic Glasses: Preparation, Characterization and Biosensor Applications

Lead ID: DMR/BMAT(7623) 0804464 Lead PI: Stanciu， Lia ORG: Purdue非铅ID: DMR/BMAT(7623) 0804506非铅PI: Andreescu， Emanuela ORG: Clarkson标题：合作研究：生物磁性玻璃：制备，表征和生物传感器应用知识优点：本提案的目标是创建一种通用方法，用于制造具有磁性的生物功能材料，即所谓的“生物磁性玻璃”。这些将用于设计高度稳定，磁分离酶为基础的系统。该项目的重点是创建包含酶、磁性纳米颗粒和硅玻璃的混合复合材料，并开发用于制备这些材料的通用策略。三种酶被选择作为模型例子：辣根过氧化物酶（HRP）、乙酰胆碱酯酶（AChE）和葡萄糖-6-磷酸脱氢酶（G6PDH）。作为拟议应用的一个例子，基于AChE的生物磁性玻璃将适合用作传感组件，用于开发廉价且自主操作的生物分析设备，用于检测食品，农业领域和其他安全情况中的农药。有人提出，传感器材料的磁性能将允许它们相对于使用永磁体的潜在分析物进行定位。该项目的具体目标是：(1)合成并表征具有可控微观结构和性能的二氧化硅-氧化铁磁性纳米颗粒。(普);(2)将模型酶（HRP, AChE, G6PDH）封装在二氧化硅磁性纳米复合材料中，并监测生物催化活性和稳定性（Purdue）；(3)利用丝网印刷电极开发生物传感器中的生物磁性玻璃（Clarkson）；(4)在应用不同浓度的目标分析物（如过氧化氢、农药、葡萄糖-6-磷酸）时建立生物传感器的校准图（克拉克森）。更广泛的影响：生物基传感器技术在广泛的动态范围内是敏感和选择性的，在生物技术过程流、环境和安全领域的化学物种分析中仍然有很大的需求。本提案特别提出了基于酶的生物传感器的使用寿命问题。该项目为研究生和本科生的多学科培养提供了良好的平台。这两个项目都鼓励本科生参与；克拉克森大学的预算中包括本科生资助。这位来自普渡大学的PI计划将有关生物传感器的材料纳入她在两所学院任教的课程中，她是这两所学院的成员。这两个pi都将参与到当地公立学校的推广活动中。在克拉克森，这些关系是建立和持续的，而普渡大学的PI正在发展与学校系统的必要关系。她还积极参与普渡大学女性工程项目，并成功地招募了女学生加入她的研究小组。两个pi都表现出对扩大代表性不足的少数民族学生的参与的敏感性。这些pi与各自校园正在进行的少数族裔招聘项目有关。

Lead ID: DMR/BMAT(7623) 0804464 Lead PI: Stanciu, Lia ORG: Purdue Non-Lead ID: DMR/BMAT(7623) 0804506 Non-Lead PI: Andreescu, Emanuela ORG: Clarkson Title: COLLABORATIVE RESEARCH: Biomagnetic Glasses: Preparation, Characterization, and Biosensor Applications INTELLECTUAL MERIT: The goal of this proposal is to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities, so called "biomagnetic glasses." These will be used in the design of highly stable, magnetically separable enzyme-based systems. The project focuses on creating hybrid composite materials containing enzymes, magnetic nanoparticles, and silica glasses and on the development of generic strategies that will be used to prepare these materials. Three enzymes have been selected as model examples: horseradish peroxidase (HRP), acetylcholinesterase (AChE), and glucose-6-phosphate dehydrogenase (G6PDH). As an example of proposed applications, the AChE based biomagnetic glasses will be suitable for use as the sensing component in the development of a cheap and autonomously operating bioanalytical device for detecting pesticides in food products, agricultural fields and other security situations. It is proposed that the magnetic properties of the sensor materials will permit them to be localized with respect to the potential analytes using permanent magnets. The specific objectives of the project are: (1) synthesize and characterize silica-iron oxide magnetic nanoparticles with controlled microstructure and properties. (Purdue); (2) encapsulate model enzymes (HRP, AChE, G6PDH) within the silica magnetic nanocomposites and monitor the biocatalytic activity and stability (Purdue); (3) use screen printed electrodes to exploit the biomagnetic glasses in biosensors (Clarkson); (4) build calibration plots of the biosensors upon application of various concentrations of target analytes (e.g. hydrogen peroxide, pesticides, glucose-6-phosphate) (Clarkson). BROADER IMPACTS: Bio-based sensor technology that is sensitive and selective across a broad dynamic range remains in great demand for analysis of chemical species in biotech process streams, in the environment, and in the security area. This proposal addresses, in particular, the issue of useful lifetime of enzyme-based biosensors. The project provides an excellent platform for multidisciplinary training of graduate and undergraduate students. Both PIs encourage undergraduate participation; funding for support of undergraduates is included in the Clarkson budget. The Purdue-based PI plans to incorporate materials on biosensors in courses that she teaches in the two Schools of which she is a member. Both PIs will engage in outreach to local public schools. At Clarkson these relationships are established and on-going, while the PI at Purdue is developing the necessary relationships with the school system. She is also active in the Purdue Women in Engineering program and has been successful in recruiting female students into her research group. Both PIs demonstrate a sensitivity toward broadening participation among underrepresented minority students. The PIs are connected with ongoing minority recruitment programs on their respective campuses.