MRI: Acquisition of a Flow Cytometer Instrument for Undergraduate Research

该奖项是根据2009年美国复苏和再投资法案(公法111-5)资助的。佩珀丁大学获得了Cell Lab Quanta SC流式细胞仪系统。流式细胞仪将用于我们的夏季本科生研究计划和荣誉的原创研究项目，学生生成的研究项目。该仪器将在生物系当前暑期计划的背景下进行的各种本科生研究项目中使用，部分资金来自NSF-REU现场拨款。购买一台流式细胞仪将使我们能够将新概念引入我们的核心细胞生物学、生物化学和分子生物学课程、几门高级生物学选修课以及运动医学和心理学的相关课程的实验室。与这项资助相关的每个教员的任务是开发一个新的本科生实验室教学模块，探索流式细胞术在细胞结构或功能中的应用。这笔助学金将为本科生提供分子和细胞生物学研究的最新设备。基金将促进细胞和分子生物学的本科生研究，以促进对细胞和分子生物学的研究生学习的兴趣，并为有兴趣在生物技术行业就业的学生提供培训。获得这台仪器将有助于开展的具体研究项目包括：(1)检测凋亡前和凋亡后细胞中的应激信号分子；(2)检测混合7F2细胞群中Notch信号的下游靶点；(3)检测神经损伤后海马区磷酸化和总CaMKII水平的变化；(4)检测正常和短期运动训练条件下哺乳动物血管平滑肌中的细胞信号；以及(5)检测瓣叶植物的倍性和杂交。此外，我们将能够将生物学本科生的研究和教学与心理学和运动医学的研究和教学联系起来，从而扩大佩珀丁大学西弗学院这些学科之间的兴趣基础和学生之间的联系。通过这笔赠款完成的研究成果将在每个调查员的网页和同行评议的出版物上公布。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Pepperdine University has acquired a Cell Lab Quanta SC Flow Cytometer system. The flow cytometer instrument will be utilized in our summer undergraduate research programs and Honor's research program in original, student generated research projects. This instrument will be utilized in a variety of undergraduate research projects carried out in the context of the biology department's current summer program funded in part through a NSF-REU site grant. Acquisition of a flow cytometer will allow us to introduce new concepts into the laboratories of our core cell biology, biochemistry and molecular biology courses, several of our upper-division biology elective courses, and related courses in sports medicine and psychology. Each faculty member associated with this grant is tasked to develop a new undergraduate laboratory teaching module that explores an application of flow cytometry in cell structure or function. The grant will provide undergraduate students with access to the latest equipment for research in molecular and cellular biology. Funds will foster undergraduate research in cell and molecular biology to facilitate interest in graduate studies in cell and molecular biology and also provide training to students interested in employment in biotechnology industries. Specific research programs that will be aided through acquisition of this instrument are: (1) detection of stress signal molecules in pre- and post-apoptotic cells; (2) examining downstream targets in Notch signaling of a mixed population of 7F2 cells; (3) examining changes in levels of phosphorylated and total CaMKII in the hippocampus following neural injury; (4) examining cellular signaling in mammalian vascular smooth muscle under normal and short term exercise training regimes; and (5) examining ploidy and hybridization in chaparral plants. In addition, we will be able to connect undergraduate research and teaching in biology with research and teaching conducted in psychology and sports medicine, thus broadening the interest base and student interconnections between these disciplines within Seaver College at Pepperdine University. Results of the research efforts accomplished from this grant will be available on each investigator's web pages and in peer reviewed publications.