Integration of Hypoxic Signaling Networks

氧气是产生维持生命所需的足够的细胞能量所必需的。因此，低氧可用性或缺氧对许多生物体（包括植物）的生存能力具有破坏性影响。氧气水平的降低可能来自植物自身对密集组织中可用氧气的消耗，或者来自环境的变幻莫测，如土壤压实，季节性洪水，甚至与耕作或灌溉有关的农业实践。在这些条件下生存需要植物感觉到氧气供应的降低，并将其能量代谢转移到系统中，以科普即将到来的能量不足。虽然这种反应对于生存至关重要，但我们目前对植物中这种感觉系统的理解非常不完整，特别是在感知和发出氧气可用性下降信号的细胞事件水平上。因此，本提案中的研究旨在更全面地表征早期事件，这些事件触发，协调和调节植物对低氧可用性的反应，使用低氧相关细胞信号传导活动的成像，基因表达相关变化的表征以及这些细胞和分子事件中受损突变体的反应分析的综合方法。除了推进植物如何适应环境的基础知识外，该提案中的研究还将与农业长期相关，因为季节性洪水可能会因气候变化而加剧，可能成为未来作物生产力的重要因素。这项基础研究还致力于开发一种植物生物学电影资源，旨在吸引和教育公众。这项工作将密切参与本科生在电影的制作和演示。

Oxygen is needed for the production of sufficient cellular energy to sustain life. Low oxygen availability, or anoxia, therefore has devastating effects on the viability of many organisms, including plants. Reduction in oxygen levels can come from the plant's own consumption of the available oxygen in densely packed tissues, or from the vagaries of the surroundings such as soil compaction, seasonal flooding and even agricultural practices related to cultivation or irrigation. Surviving these conditions requires the plant to sense the lowering of oxygen availability and shift its energy metabolism to systems to cope with the impending energy deficit. Although this response is essential for survival, our current understanding of this sensory system in plants is highly incomplete, especially at the level of the cellular events that sense and signal a fall in oxygen availability. Therefore, the research in this proposal seeks to more fully characterize the early events that trigger, coordinate and regulate plant responses to low oxygen availability using an integrated approach of imaging of low oxygen-related cellular signaling activities, characterization of associated changes in gene expression and analysis of the responses of mutants impaired in these cell and molecular events. In addition to advancing fundamental knowledge of how plants adapt to the environment, the research in this proposal will also be of long-term relevance to agriculture where seasonal flooding, likely exacerbated by climate change, may become a significant factor in future crop productivity. Integrated into this basic research is also an effort to develop a plant biology movie resource aimed at engaging and educating the general public. This effort will intimately involve undergraduate students in the making and presentation of the movies.