Confocal microscopy at Hopkins Marine Station

斯坦福大学获得了一笔赠款，用于购买共焦扫描激光显微镜，并在霍普金斯海洋站(HMS)建立相关的多用户设施，为加利福尼亚州蒙特利湾半岛的多个海洋实验室提供服务。高分辨率成像现在是当代细胞和发育生物学过程工具包的重要组成部分。随着蛋白质和核酸荧光标记技术的进步，共聚焦显微镜使人们对许多海洋生物的细胞和组织的三维结构和功能有了前所未有的了解。这项技术的一个重要应用是可视化与早期发育、生理学和神经生物学相关的细胞元素和过程。这一技术能力对于阐明海洋动物与其环境的相互作用也具有更广泛的影响。最终，环境通过对细胞和组织的影响作用于有机体。海洋酸化、除氧和其他过程将通过对个别生物体的影响而改变生态系统和渔业。该显微镜将帮助解决气候变化的各个方面如何影响各种海洋物种的细胞/发育生物学和生理学的关键方面。这一设备将直接惠及HMS的5个研究实验室，并立即惠及10名研究生，并在研究培训学徒期间提供出色的本科生培训机会。显微镜将被安置在HMS，但当地其他研究机构的研究人员和他们的学生也可以使用，包括蒙特利湾水族馆、蒙特利湾水族馆研究所和加州州立大学蒙特利湾研究所(CSUMB)。这些机构的许多教职员工都有广泛的研究兴趣，他们计划在有货的情况下使用这种显微镜。HMS将与CSUMB Bay合作，为麦克奈尔奖学金计划创造这种显微镜的培训机会，为有天赋的少数族裔学生做好博士学习的准备。该设备产生的图像数据将在HMS的定期公开讲座和开放参观中突出显示，并将在致力于海洋无脊椎动物早期胚胎发育和幼体发育的网络资源上突出显示。HMS和蒙特利湾水族馆(MBA)之间的成像合作将通过在正式公开展品和Facebook帖子中使用MBA广泛建立的扩展机制来突出图像数据，从而提高公众对海洋无脊椎动物胚胎和幼虫多样性的认识。欲了解更多有关霍普金斯海洋站的信息，请访问网站：http://www-marine.stanford.edu/.。

Stanford University has been awarded a grant to purchase a confocal scanning laser microscope and establish a related multi-user facility at Hopkins Marine Station (HMS) to serve multiple marine laboratories in the Monterey Bay Peninsula, California. High-resolution imaging is now an essential part of the contemporary tool kit of cell and developmental biological processes. Confocal microscopy, along with advances in fluorescent labeling of protein and nucleic acids, has resulted in unprecedented insights into the 3D architecture and function of cells and tissues of many marine organisms. An important application of this technology is visualization of cellular elements and processes related to early development, physiology, and neurobiology. This technical ability also has broader implications for elucidating the interactions of marine animals with their environment. Ultimately, the environment acts on organisms through its effects on cells and tissues. Ocean acidification, deoxygenation and other processes will alter ecosystems and fisheries through effects on individual organisms. This microscope will help address how the various facets of climate change are impacting critical aspects of the cell/developmental biology and physiology of a variety of marine species. This piece of equipment will directly benefit 5 research labs at HMS with an immediate potential benefit to 10 graduate students, and provide outstanding undergraduate training opportunities during research training apprenticeships. The microscope will be housed at HMS but be available to researchers and their students at other research institutions in the local area, including the Monterey Bay Aquarium, Monterey Bay Aquarium Research Institute at Moss Landing and California State University Monterey Bay (CSUMB). There are many faculty at these institutions with a wide range of research interests who plan to use this microscope upon availability. HMS will partner with CSUMB Bay to create training opportunities on this microscope for the McNair Scholars program for gifted minority students to prepare them for doctoral studies. The image data generated from this equipment will be highlighted in the regular public lectures and open houses at HMS, and on a web-based resource dedicated to early embryogenesis and larval development of marine invertebrates. An imaging collaboration between HMS and the Monterey Bay Aquarium (MBA) will develop a greater public awareness of the diversity of embryos and larvae of marine invertebrates by spotlighting image data using the extensive established outreach mechanisms of the MBA both in formal public exhibits and Facebook postings. For more information about Hopkins Marine Station, visit the website at http://www-marine.stanford.edu/.