Towards a Plant Nuclear Envelope Interactome

植物核膜蛋白在许多细胞功能中起着重要的作用，包括机械信号，控制细胞核的形状和运动，以及细胞周期。然而，由于目前尚不清楚的原因，植物核膜的蛋白质组成与动物和真菌的蛋白质组成有很大不同。该项目的目标是了解跨越核膜的新型植物特异性蛋白质的功能，这些蛋白质被预测在植物发育，授粉以及对光和病原体的反应中发挥作用。实验方法将包括通过光学显微镜进行活细胞成像，以及使用亲和层析、串联质谱和计算生物学技术鉴定蛋白质相互作用伙伴。更广泛的影响。我们将与俄亥俄州立大学的一位艺术教授合作，开设一门关于生物学和艺术的新课程，目的是让公众能够了解和理解当代植物生物学的主题。一个公共的生物艺术展览将被开发出来，课程将成为一个模块的基础，可以被高中教师使用。

The proteins of the plant nuclear envelope have important roles in many cell functions, including mechanical signaling, controlling the shape and movement of the nucleus, and the cell cycle. However, for reasons that are not currently understood, the protein composition of the plant nuclear envelope differs significantly from that of the animals and fungi. The goal of this project is to understand the function of the novel plant-specific proteins that span the nuclear envelope, and which are predicted to play roles in plant development, pollination, and the responses to light and pathogens. The experimental approaches will include live-cell imaging by light microscopy, and the identification of the protein interaction partners using techniques of affinity chromatography, tandem mass spectrometry and computational biology. Broader Impacts. A novel course in biology and art will be developed, in collaboration with an Art professor at Ohio State University, with the goal to make topics in contemporary plant biology accessible and comprehensible to the public. A public Bio-Art exhibit will be developed and the course will form the basis for a module that can be used by high school teachers.