CAREER: Plasmonic Nanoclusters with Built-in Artificial Antibodies for Label-free Biosensing

1254399 - SingamaneniPlasmonic传感器在开发低成本、无标签、即时生物诊断、快速化学检测和环境监测方面具有巨大潜力。然而，需要克服两个主要挑战以获得这类生物传感器的益处：（i）现有技术的等离子体生物传感器要么提供有限的灵敏度，要么由于其差的稳定性和过高的成本而对于现实世界的应用是不切实际的;（ii）这些生物传感器依赖于天然抗体，其是高成本的并且在处理、存储和设备集成方面施加了严重的限制。我们建议实现高灵敏度和廉价的等离子体传感器。拟议工作的重点将是化学合成等离子体纳米结构的分级有序的自组装阵列。我们将结合人工单克隆抗体，并证明这些是一个高度敏感，特异性，稳定和低成本的生物诊断平台。这将通过定向控制的分子印迹来实现，这一建议将导致更简单和更强大的生物传感器的发展，用于检测多种重要的化合物。 这将通过用基于更便宜和更稳定材料的人工等效物取代抗体-复杂，昂贵和不稳定的分子来实现。 此外，新的纳米技术方法将用于检测。

1254399 - SingamaneniPlasmonic sensors hold enormous potential for the development of low-cost, label-free, point-of-care biodiagnostics, rapid chemical detection and environmental monitoring. However, two major challenges need to be overcome to reap the benefits of this class of biosensors: (i) state-of-theart plasmonic biosensors either offer limited sensitivity or are impractical for real-world applications due to their poor stability and excessive cost; (ii) these biosensors rely on natural antibodies, which are high-cost and impose severe limitations in handling, storage and device integration. We propose to realize highly sensitive and inexpensive plasmonic sensors. The focus of the proposed effort will be hierarchically ordered, self-assembled arrays of chemically synthesized plasmonic nanostructures. We will incorporate artificial monoclonal antibodies and demonstrate these to be a highly sensitive, specific, stable and low-cost biodiagnostic platform. This will be achieved through orientation-controlled molecular imprinting.This proposal will lead to development of simpler and more robust biosensors for detection of multiple important compounds. This will be achieved by replacing antibodies - complicated, expensive and unstable molecules - with artificial equivalents based on less expensive and more stable materials. In addition, novel nanotechnology approaches will be used for detection.