MRI: Acquisition of a Flow Sorter Cytometer to Advance Marine Research and Education

PI们要求提供资金，以获得最先进的细胞分选流式细胞仪。流式细胞术的使用已经成为微生物海洋学中大多数研究的核心，并在评估种群组成和功能变化的“适应性”采样中至关重要。所要求的仪器是一种模块化、多功能、高速的细胞分选器，它将能够识别、计数和分类光学特征有细微差异的细胞。该仪器的一个创新之处在于，它对识别和分类光学特征有细微差异的生物所需的探测器进行了无与伦比的手动调整，这在微生物海洋学研究中至关重要，这些研究涉及多样性、生态系统功能、群落组成变化的生物地球化学效应以及对海洋微生物种群的进化。更广泛的影响：拟议的流式细胞仪设施不仅将显著促进海洋科学研究，而且将可供工程学、医学和人类学同事使用。它将用于为本科生和研究生开设的新班级，以及将增加UCSB人员与其他研究人员之间合作的年度研讨会。通过媒体采访和与COSEE和学校团体的互动，PI在提供访问方面有着良好的记录。该设施的一个主要价值将是开设一门新的本科生和研究生课程：“流式细胞术在现代微生物海洋学中的应用”。

The PIs request funding to acquire a state-of-the-art cell-sorter flow cytometer. The use of flow cytometry has become central to most research in microbial oceanography, and critical in 'adaptive' sampling to assess shifts in population composition and function. The requested instrument is a modular, versatile, highspeed cell sorter that will enable the identification, enumeration and sorting of cells with subtle differences in their optical signatures. A novelty of the instrument is its unparalleled manual adjustment of detectors required to identify and sort organisms with subtle differences in their optical signatures, which is central in microbial oceanographic studies dealing with diversity, ecosystem function, biogeochemical effects of shifts in community composition, and evolution on marine microbial groups. Broader Impacts: The proposed flow cytometry facility will not only significantly further marine science research but will be available to engineering, medical, and anthropological colleagues. It will be used in a new class for undergraduate and graduate marine science students and in yearly workshops that will increase collaboration between UCSB personnel and other researchers. The PI has a good track record of providing access through media interviews and interactions with COSEE and school groups. A major value of the facility will be the creation of a new undergraduate and postgraduate course: 'Flow Cytometry Applications in Modern Microbial Oceanography'.