Hydrodynamics of Self-Propelled Deformable Cells

PI: Bagchi， ProsenjitProposal Number: 1438255这项被提议的工作的目标是研究生物细胞的推进力，利用它们极端可变形的膜来移动。细胞可以通过细胞膜特定方向的内部流动来变形和延伸一个突起，称为假足，假足用于移动细胞。本文从流体力学的角度出发，结合高保真计算建模、理论分析和实验测量，研究可变形细胞的伪足推进。在对健康的影响方面，该项目可能提供如何抑制恶性细胞推进机制的机制理解。虽然最近在植物细胞中进行了流体力学分析，但这些细胞是非变形和非推进性的，但在变形细胞中，流体流动，假足动力学和运动活动之间的关系尚不清楚。co- pi建议开发由分子马达驱动的界面变形的多尺度三维计算模型。该模型将同时预测动态细胞形状、流场和运动活动。同时，将在活的变形虫细胞中进行微piv实验，提取双分量的准瞬时细胞内速度场。通过对模拟和实验数据的分析，将探讨变形细胞中流动的性质和起源，以及它与伪足动力学的关系。该奖项由CBET部流体动力学项目颁发，由数学科学部计算数学项目共同资助。

PI: Bagchi, ProsenjitProposal Number: 1438255The objective of the proposed work is to study the propulsion of biological cells that utilize their extremely deformable membrane to move. Cells can deform and extend a protrusion, called pseudopod, by streaming flow internally in a particular direction of the cell membrane, and the pseudopod is used to move the cell. This is a proposal to study the pseudopod-based propulsion of deformable cells from a fluid mechanical viewpoint using a combination of high-fidelity computational modeling, theoretical analysis and experimental measurements. In terms of impact on health, the project may provide a mechanistic understanding of how to inhibit propulsive mechanisms of malignant cells.While fluid mechanical analysis of the streaming flow has been carried out recently in plant cells, which are non-deforming and non-propulsive, the relationship between the streaming flow, pseudopod dynamics, and motor activity is unknown in deformable cells. The co-PIs propose to develop a multiscale, 3D computational model of the interface deformation driven by molecular motors. The model will predict simultaneously the dynamic cell shape, the flow field, and motor activity. In parallel, micro-PIV experiments will be performed in live amoeboid cells to extract the 2-component quasi-instantaneous intra-cellular velocity field. Using analysis of the simulation and experimental data, the nature and origin of the streaming flow in deforming cells, and its relation to pseudopod dynamics will be explored.This award by the Fluid Dynamics Program of the CBET Division is co-funded by the Computational Mathematics Program in the Division of Mathematical Sciences.