New Methods for Structural Biology in Solution

该项目旨在扩大核磁共振光谱在制药研究中的应用范围，在制药研究中，核磁共振光谱已经被常规用于鉴定与蛋白质靶标结合的化合物。开发的技术旨在提供快速和广泛适用的工具，用于确定与其蛋白质靶标结合的化合物的3D结构，识别蛋白质-蛋白质相互作用部位，以及表征蛋白质运动。核磁共振光谱学的极限将被推向分析分子量显著增加的体系。该项目包括应用于药物靶标，如登革热病毒NS2B/NS3蛋白酶。

This project aims to expand the range of applications of nuclear magnetic resonance (NMR) spectroscopy in pharmaceutical research, where NMR spectroscopy is already used routinely for the identification of chemical compounds that bind to protein targets. The techniques developed aim at providing rapid and broadly applicable tools for 3D structure determinations of chemical compounds bound to their protein target, identification of protein-protein interaction sites and characterization of protein motions. The limits of NMR spectroscopy will be pushed to analyse systems of significantly increased molecular weights. The project includes applications to drug targets such as the dengue virus NS2B/NS3 protease.