3D C-NEMS Based Aptasensors

标题：基于3DC-NEMS的适配传感器项目目标简介：设计、制造、评估和优化基于碳纳米机电系统(C-NEMS)的三维芯片上电化学适配传感器。非技术摘要：在早期肿瘤发生和癌症进展中可以观察到诸如血小板衍生生长因子(PDGF)等蛋白质的增加。PI将专注于开发使用适体作为识别元件的片上电容式适配传感器，使用独特的基于三维石墨烯的微柱阵列，可以提供高灵敏度、良好的稳定性和低检测限。小型化的可重构电极设计是为了利用界面面积和总体积之间的可伸缩关系。该项目将为研究、教育和多样性目标做出贡献，并大力支持FIUBeyondPasible2020战略计划。它将扩大少数族裔学生和妇女对科学和工程的参与，并促进跨学科互动。除了对微制造研究界的影响外，该项目还将对癌症诊断产生更广泛的影响。该项目中产生的知识和确定的关键问题将影响传感器制造和开发的广泛领域。技术摘要：在早期肿瘤发生和癌症进展中，可以观察到血小板衍生生长因子(PDGF)等蛋白质的增加。在这个项目中，PI将专注于开发基于独特的3D C-MEMS(碳微电子机械系统)和C-NEMS(碳纳米机电系统)平台的使用适配子作为识别元件的片上适配传感器。本项目的目标是设计、制造、评估和优化基于片上电化学适配传感器的3D C-NEMS，以提供优异的性能(即高灵敏度、良好的稳定性、低检测限)。PI计划：(1)利用C-MEMS技术制备具有交指结构的3D大纵横比微电极阵列；(2)采用静电喷射沉积和电泳沉积的方法在C-MEMS上沉积碳基纳米材料；(3)将带有活性化学基团的全碳平台功能化，以实现高效、稳定的适配子的固定化；(4)基于电化学阻抗和电容行为的表征，研制并评价了一种用于PDGF蛋白质检测的无标记电化学适体传感器。3D C-NEMS平台将具有很大的优势，是实现高灵敏度生物传感器的最佳解决方案之一。采用独特的C-NEMS技术，可以重复制造具有所需尺寸、结构和材料特性的适配子传感器。微型化的电极设计是为了利用界面面积和总体积之间的可伸缩关系。该项目将为研究、教育和多样性目标做出贡献，并大力支持FIUBeyondPasible2020战略计划。它将扩大少数民族学生和妇女对科学和工程的参与。这项新开发的技术将在PI的研究生/本科课程中得到强调。除了对学术界的MEMS和NEMS社区的影响外，拟议的研究还将对癌症诊断产生更广泛的影响。该项目中产生的知识和确定的关键问题将影响传感器制造和开发的广泛领域。预计该设备在临床领域的成功实施将对大量癌症患者的早期发现、发病率和死亡率产生重大影响，并建立生物医学技术的创新领域。

Title: 3D C-NEMS Based AptasensorsBrief description of project Goals: Design, fabricate, evaluate and optimize three dimensional on-chip electrochemical aptasensors based on Carbon-NanoElectroMechanical Systems (C-NEMS).Nontechnical Abstract:The increased amount of proteins such as platelet-derived growth factor (PDGF) can be observed in the early tumorigenesis as well as cancer progression. The PI will focus on developing on-chip capacitive aptasensors employing aptamers as recognition element using unique three dimensional graphene based micropillar arrays, which could deliver high sensitivity, good stability and low detection limits. The miniaturized reconfigurable electrode design is geared to take advantage from the scalable relationship between the interfacial area and overall volume. This project will contribute to the research, education, and diversity goals and strongly support FIUBeyondPossible2020 strategic plan. It will broaden the participation of minority students and women in science and engineering, and foster interdisciplinary interactions. Beyond the impact on the microfabrication research community, this project will have broader impact on cancer diagnosis. The knowledge generated and the key issues identified in this project will impact a broad area of sensor fabrication and development.Technical Abstract:The increased amount of proteins such as platelet-derived growth factor (PDGF) can be observed in the early tumorigenesis as well as cancer progression. In this project, the PI will focus on developing on-chip aptasensors employing aptamers as recognition element based on unique 3D C-MEMS (Carbon-MicroElectroMechanical Systems) and C-NEMS (Carbon-NanoElectroMechanical Systems) platforms. The goal of this project is to design, fabricate, evaluate and optimize the 3D C-NEMS based on-chip electrochemical aptasensors that deliver superior performance (i.e., high sensitivity, good stability, low detection limits).The PI plans to (1) fabricate 3D high-aspect-ratio microelectrode arrays with interdigital fingers by C-MEMS technique; (2) deposit carbon based nanomaterials onto the C-MEMS using electrostatic spray deposition and electrophoretic deposition; (3) functionalize the whole-carbon platform with active chemical groups for efficient and stable immobilization of aptamers; (4) develop and evaluate an electrochemical label-free aptasensor for PDGF protein detection based on characterizing electrochemical impedance and capacitive behaviors. 3D C-NEMS platform will have great advantages and is one of the best solutions to achieve high-sensitivity biosensors. Employing unique C-NEMS technique aptasensors can be fabricated repeatedly with desired dimensions, structures, and material properties. The miniaturized electrode design is geared to take advantage from the scalable relationship between the interfacial area and overall volume. This project will contribute to the research, education, and diversity goals and strongly support FIUBeyondPossible2020 strategic plan. It will broaden the participation of minority students and women in science and engineering. The newly developed technique will be highlighted in the PI's graduate/undergraduate courses. Beyond the impact on the MEMS and NEMS community in academia, the proposed research will have broader impact on cancer diagnosis. The knowledge generated and the key issues identified in this project will impact a broad area of sensor fabrication and development. It is anticipated that the successful implementation of this device in the clinical arena will have significant impact on the early detection, morbidity, and mortality for the large number of patients with cancers, as well as establishing an innovative realm of biomedical technology.