Infrared Laser Ablation Microsampling for Mass Spectrometry Imaging

该项目由化学部的化学测量和成像计划资助。路易斯安那州立大学的Kermit Murray教授和贝勒大学的Touradj Solouki教授正在开发观察(成像)生物组织的技术，以便识别和量化样本中的生物分子。组织成像通常局限于最丰富的化合物，无法识别重要但不那么丰富的分子，这些分子可能是生物功能和疾病诊断的关键因素。该项目使用激光对组织进行直接采样，并使用精密显微解剖进行详细分析。定位、识别和量化组织中的生物分子的能力对基础研究和临床诊断非常重要--特别是当监测治疗进展很重要的时候。外展计划包括一项新颖的开放式教育计划，利用本研究开发的可视化工具创建基于网络的教育资源。教育机会提供给高中生、本科生和研究生。路易斯安那州立大学在为代表不足的学生，特别是非裔美国人提供优质导师方面有着很强的历史。这种质量指导在这项工作中得到了延续和推进。这个项目的目的是开发一种全面的、定量的、能够识别生物分子的质谱学成像方法。将基质辅助激光解吸电离质谱仪成像与红外激光烧蚀样品转移相结合，用于液-质联用质谱分析。目标是构建红外激光消融组织微探针，将MALDI成像与激光消融样品传输相结合，并研究组织激光消融的基本过程以实现技术优化。教育目标支持路易斯安那州立大学和贝勒分校的研究生培训，并促进这些机构之间的合作互动。这项工作通过开发一种广泛适用的方法在分子水平上进行生物成像并加以应用，从而产生了社会效益。该项目的教育和推广部分涉及为质谱学教育和研究开发开放资源。这些组成部分包括为维基百科上的质谱学创建协作资源，以及将人类蛋白质组组织(HuPo)控制的词汇与国际纯化学和应用化学联合会开发的术语进行整合。

This project is funded by the Chemical Measurement and Imaging program of the Chemistry Division. Professors Kermit Murray of Louisiana State University (LSU) and Professor Touradj Solouki of Baylor University are developing techniques for looking at (imaging) biological tissue in order to identify and quantify the biomolecules in the samples. Tissue imaging is often limited to the most abundant compounds and cannot identify important but less abundant molecules that may be key factors for biological function and disease diagnostics. This project uses lasers for direct sampling of tissue as well as precision microdissection for detailed analysis. The ability to localize, identify, and quantify biomolecules in tissue is important to fundamental research and to clinical diagnostics - especially when it is important to monitor the progress of treatment. The outreach program includes a novel, open education initiative creating Web-based resources for education using the visualization tools developed in this research. Educational opportunities are provided to high school, undergraduate, and graduate students. LSU has a strong history of quality mentorship for underrepresented students, especially African Americans. That quality mentorship is continued and advanced in this work. This project is aimed at the development of a comprehensive approach to mass spectrometry imaging that is quantitative and capable of biomolecule identification. Matrix-assisted laser desorption ionization mass spectrometry imaging is combined with infrared laser ablation sample transfer for liquid chromatography tandem mass spectrometry analysis. The objectives are construction of an infrared laser ablation tissue microprobe, combining MALDI imaging with laser ablation sample transfer, and investigation of the fundamental processes of tissue laser ablation for technique optimization. The educational goals support graduate student training at LSU and Baylor and is promoting collaborative interactions between these institutions. This work has societal benefits through the development of a broadly applicable approach to biological imaging at the molecular level with applications. Education and outreach components of this project involve the development of open resources for mass spectrometry education and research. These components include creating collaborative resources for mass spectrometry on Wikipedia and integration of Human Proteome Organization (HUPO) controlled vocabulary with terminology developed by the International Union of Pure and Applied Chemistry.