Mechanically stable anti-adhesive polymer surfaces by biomimetic structuring

避免细菌沉降对于应用于生物医学技术的材料非常重要。模仿自然发生的表面为满足这一要求提供了有希望的解决方案。从弹尾的不润湿、抗粘接和机械稳定的皮肤出发，我们建议应用纳米复制和激光干涉光刻技术来创建具有相似特性的聚合物表面。包含角质层分层形貌的纳米复刻剂将被用于研究纳米级结构元素和化学表面成分对其抗粘接行为的影响。激光干涉光刻技术以不同的尺寸和形状再现皮肤的单个结构元素的阵列。应定量分析结构聚合物表面的特性，包括润湿、蛋白质吸附和不同类型细菌的沉降。应对细菌的初始粘附（例如，使用粘附力测量，结合细菌基质分子成分的检测）和在结构材料上的定植进行详细调查，以得出影响细菌在人工表面粘附的因素的一般结论。在此基础上，将开发出原则性的解决方案，以扩大医疗器械中应用材料的有效表面结构。

Avoiding bacterial settlement is important for materials applied in biomedical technology. Mimicking naturally occurring surfaces offers promising solutions to meet this requirement. Starting from the non-wetting, anti-adhesive and mechanically stable skin of collembola (springtails) we propose to apply nanoreplication and laser interference lithography to create polymer surfaces with similar characteristics. Nanoreplicas that contain the hierarchical topography of the cuticle shall be applied to investigate the impact of the nanoscale structural elements and the chemical surface composition on their anti-adhesive behavior. Laser interference lithography shall be used to recapitulate arrays of individual structural elements of the skin in varying dimensions and shapes. The characteristics of the structured polymer surfaces shall be quantitatively analyzed with respect to wetting, protein adsorption and settlement of different types of bacteria. Detailed investigations of the initial bacterial adhesion (using, e.g., adhesion force measurements, combined with the detection of molecular components of the bacterial matrix) and colonization on the structured materials shall be performed to draw general conclusions on factors that influence bacterial adhesion at artificial surfaces. Based on that, principle solutions shall be developed to scale up effective surface structures for materials applied in medical devices.