CSBR: Living Stocks: The Escherichia Coli Genetic Stock Center

该奖项将为耶鲁大学的大肠杆菌遗传资源中心(CGSC)的运作做出贡献。大肠埃希氏菌是一种重要的模式生物，从遗传学和生物化学的角度来看，可以说是最完全被理解的细胞生物。这种高度的理解使大肠杆菌不仅成为一个重要的研究对象，而且成为研究各种生物的实验室主力，以及生物技术的无价工具。CGSC的使命是充当由研究界创造的转基因大肠杆菌菌株的中央储存库，以便其他研究人员和教育工作者可以随时获得这些菌株。为此，CGSC保留了大约28,000种由基因定义的E.Coli K-12衍生物的培养物。该菌株集合中有超过8,000种不同的突变，在已测序的大肠杆菌K-12基因组中的4,111个确定的基因中，超过90%的基因被证实存在突变。这些文化被分发给全美和全球65多个国家的研究人员和教育工作者。除了文化的分布，CGSC也是一个信息资源，在网上一个完全可搜索的数据库中提供关于菌株、突变、基因、基因产物和基因图谱的信息。重要的是，CGSC还为那些对在收集中使用大肠杆菌菌株有疑问的研究人员提供专家信息和建议的来源。CGSC将继续维护和扩大其关于菌株基因类型、表型、病史和系谱的公开数据库，这是库存中心独有的。信息系统将得到加强，以增加与其他大肠杆菌网络资源的兼容性，包括EcoliWiki项目、Ecogene和EcoCyc。CGSC正在与其他美国培养物收藏品和世界微生物数据中心(WDCM)合作，以便这些收藏品可以由全球菌株数据库编制索引，并创建一个国家微生物菌株数据库。在获奖期间，我们的新菌株构建服务的能力将扩大到那些需要转基因菌株，但缺乏自己构建它的能力的用户，特别是那些传统细菌遗传学社区之外的用户。这是CGSC独有的一项服务，并显示出巨大的增长潜力。对菌株收集数据库的访问，包括基因类型、基因功能和图谱位置，以及广泛的参考书目，都可以在网上获得(cgsc.biology.yale.edu)。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This award will contribute to the operation of the E. coli Genetic Stock Center (CGSC) at Yale University. Escherichia coli is an important model organism and is arguably the most completely understood cellular organism from a genetics and biochemistry standpoint. This high degree of understanding has made E. coli not just an important subject of research, but also the laboratory workhorse for studying a broad variety of organisms as well as an invaluable tool for biotechnology. The mission of the CGSC is to act as a central repository for genetically modified strains of E. coli created by the research community, so that they may be readily available to other researchers and educators. To that end, the CGSC maintains a collection of about 28,000 cultures of genetically defined derivatives of E. coli K-12. There are over 8,000 different mutations represented in this strain collection with verified mutations in over 90% of the 4,111 identified genes in the sequenced E. coli K-12 genome. These cultures are distributed to researchers and educators throughout the US and in over 65 countries worldwide. In addition to the distribution of cultures, the CGSC is also an information resource, and provides information about strains, mutations, genes, gene products and genetic maps, in a fully searchable database on the web. Importantly CGSC also acts as a source of expert information and advice for those researchers who have questions about using the E. coli strains in the collection. The CGSC will continue to maintain and expand its publicly available database on strain genotypes, phenotypes, histories and pedigrees, which is unique to the Stock Center. The information systems will be enhanced to increase compatibility with other E. coli web resources, including the EcoliWiki project, EcoGene and EcoCyc. The CGSC is collaborating with other US culture collections and the World Data Centre for Microorganisms (WDCM) so that the collection can be indexed by global strain databases and to create a national microbial strain database. Over the period of this award, capabilities for our new strain construction services will be expanded for those users who require a genetically modified strain, but lack the capability of constructing it themselves, particularly to those outside of the traditional bacterial genetics community. This is a service unique to the CGSC and has shown great growth potential. Access to the strain collection database, including genotypes, gene function and map location, as well as an extensive bibliography are available online (cgsc.biology.yale.edu).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.