In vivo und in vitro investigations on the effect of endemic porcine intestinal yeast, Kazachstania slooffiae, on gut microbiota and performance of weaning piglets

断奶可以被认为是猪生命周期中最关键的时期之一，往往与健康障碍和高经济损失有关。因此，肠道微生物区系对动物健康至关重要。初步试验表明，仔猪饲喂固体饲料后，肠道中主要是酵母菌Kazachstania slooffiae，但其底物谱尚不清楚。这种酵母特别令人感兴趣，因为它似乎与乳杆菌正相互作用，而与肠道细菌负相互作用，这提供了一种保护潜力。该项目的目的是检查断奶仔猪的肠道是否可以有目的地被斯洛菲克雷伯菌定植，以及这种酵母菌是否会影响猪肠道微生物区系。在为期4周的实验中，采用454测序技术对口服斯氏克雷伯氏菌后的健康状况、动物技术参数和肠道微生物区系组成进行了测定。该酵母将通过其全基因组和代谢物分析进行全面的特征分析。在体外进行研究，以检验酵母菌发酵不同底物的能力，从而检测形成的产物。

Weaning can be regarded as one of the most critical periods in the life cycle of pigs and is often related with health disorders and high economic losses. The intestinal microbiota is thereby of crucial importance for the animal health. It was shown in preliminary tests the yeast Kazachstania slooffiae dominates the gut of piglets after intake of solid feed, whereas it is not clear yet its substrate spectrum. This yeast is of special interest, because it seems to interact positively with lactobacilli and negatively with enterobacteria, which offers a protective potential. The aim of the project is to examine whether the intestine of weaning piglets can be purposefully colonized with K. slooffiae and whether this yeast can influence porcine intestinal microbiota. In a 4 weeks experiment, health status, zootechnical parameters and the composition of intestinal microbiota, using 454 sequencing technology, are determined after oral application of K. slooffiae. This yeast will be comprehensively characterized by means of its whole genome and metabolites analyses. In vitro studies are performed to examine the ability of the yeast to ferment different substrates and thereby formed products will be examined.