CSBR: Living Stocks: Provasoli-Guillard National Center for Marine Algae and Microbiota

微藻是必不可少的光合植物，支持几乎所有水生食物网的多样化生态，在全球环境和调节全球气候中发挥核心作用，最近还支持快速增长的全球生物经济。国家海洋藻类和微生物群中心（NCMA）是全球为数不多的专注于培养藻类生物多样性的机构之一，也是美国唯一一个主要关注海洋微藻的机构。NCMA从世界各地分离出3000多种不同的藻类菌株，代表了目前科学界已知的所有类别的海洋微藻。NCMA收集的许多藻类是该特定收集所独有的，因此代表了全球社区不可替代的遗传资源。近四十年来，NCMA为全世界的研究人员、各级学生、教育工作者和公司提供了这一独特的资源，他们都对微藻研究生物学、全球过程和生物技术中的基本问题感兴趣。作为NCMA对研究界重要性的一个例子，每年有200-300篇发表的论文引用了使用NCMA策划的微藻菌株，其中许多论文得到了美国国家科学基金会和其他美国联邦机构的支持。该奖项将支持NCMA文化收藏及其活动的明显改进，并为我们的各种利益相关者团体提供附加价值。该奖项将使NCMA能够1)继续最大限度地提高海洋浮游植物的生物多样性；2)完善低温保存的程序和配方，作为微藻收藏的长期管理策略；3)整合成像细胞术作为我们的收集维护和分配程序的一部分，以提供与每个菌株相关的增强价值；4)扩大微藻教育培训组合。该奖项的具体成果将包括：继续向利益相关者提供专业策划的微藻培养和培养支持服务的文件，加入任何新发现的微藻以支持当前和未来的科学，以及扩展我们与每个加入的菌株相关的公共可搜索信息数据库，以包括新的科学信息。此外，该奖项将支持纳入相对较新的仪器技术，成像流式细胞术，以便轻松生成关于细胞丰度和状况的基本报告，包括嵌入图像，为我们分发的每个微藻菌株提供。这些报告将为利益攸关方提供非常需要的信息，说明他们订购的菌株的预期情况。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Microalgae are essential photosynthetic plants that support the diverse ecology of nearly all aquatic food webs, play a central role in the global environment and regulating global climate, and more recently support a rapidly growing global bioeconomy. The National Center for Marine Algae and Microbiota (NCMA) is one of a small number of global culture collections focused on curating algae biodiversity, and the only one in the U.S. with a primary focus on marine microalgae. The NCMA curates over 3,000 distinct algal strains isolated from around the world representing all classes of marine microalgae currently known to science. Many of the algae in the NCMA Collection are unique to this specific collection and thus represent an irreplaceable genetic resource for the global community. For nearly four decades, the NCMA has provided this unique resource to researchers, students at all levels, educators and companies worldwide, all interested in working with microalgae to investigate fundamental questions in biology, global processes and biotechnology. As an example of the importance of the NCMA to the research community, each year, 200-300 published papers cite use of NCMA-curated microalgae strains, with many of these papers being supported by the NSF and other U.S. federal agencies. This award will support demonstrable improvements to the NCMA Culture Collection, its activities, and provide added value to our various stakeholder groups. This award will allow the NCMA to 1) continue to maximize curated biodiversity of marine phytoplankton; 2) refine the procedures and formulations for cryopreservation as a long term management strategy for microalgae collections; 3) integrate imaging cytometry as part of our collection maintenance and distribution procedures to provide enhanced value related to each strain; and 4) expand a microalgae education and training portfolio. Specific deliverables from this award will be documentation of the continued provision of professionally curated microalgae cultures and culture support services to stakeholders, the accession of any newly discovered microalgae for support of present and future science, and expansion of our publically searchable informational database associated with each accessioned strain to include new scientific information. In addition, this award will support the inclusion of relatively new instrument technology, imaging flow cytometry, to allow for the easy generation of basic reports on cell abundance and condition, including embedded images, to be provided with each microalgae strain we distribute. These reports will provide stakeholders much needed information on what to expect for the strains they have ordered.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.