MRI: Acquisition of a Flow Cytometer Cell Sorter for Multidisciplinary Studies at Oakland University

奥克兰大学获得了一项奖励，以获得一台荧光活化细胞分选（FACS）机器，以支持和加强该机构的多学科研究和教育计划。FACS机器将允许本科生/研究生和教师参与研究和教育活动，这将增强生物科学系和化学系以及眼科研究所的使命。具体来说，FACS机器将允许在两栖动物生物学、癌症生物学、化学、DNA修复、发育生物学、免疫学、分子生物学、植物基因组学和干细胞生物学等领域进行动手合作研究。该仪器将被纳入科学和生物工程课程，作为教学实验室和设计项目的一部分，并将允许新的课程开发。该仪器还将允许学生和教师招聘，并为周边地区提供先进的服务技术。流式细胞术与细胞分选相结合，通过分析细胞表面和细胞内分子的表达，可以表征、定义和分选不同的细胞类型和亚群。在一些特定的项目中，该仪器将被用于干细胞中由细胞表面蛋白指导的基本生物学功能和信号的测定，内皮细胞在抗凋亡途径中的作用，植物中pre-mRNA加工的调节，温度对两栖动物免疫学的影响，管状器官的发育，以及对DNA修复机制的基本了解。使用该仪器的研究结果将在同行评议的出版物、科学会议上传播，并通过本机构教师参加的外展活动向社区传播。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

An award is made to Oakland University to acquire a Fluorescence-Activated Cell Sorting (FACS) machine to support and enhance multidisciplinary research and educational programs at the institution. The FACS machine will allow undergraduate/graduate students and faculty to participate in research and educational activities that will enhance the mission of the Departments of Biological Sciences and Chemistry as well as the Eye Research Institute. Specifically, the FACS machine will allow for hands-on collaborative research in the areas of amphibian biology, cancer biology, chemistry, DNA repair, developmental biology, immunology, molecular biology, plant genomics, and stem cell biology. The instrument will be incorporated into the science and bioengineering curriculums, as part of teaching laboratories and design projects, and will allow for new course development. This instrument will also allow for student and faculty recruitment and provide a sophisticated service technology to the surrounding region.Flow cytometry combined with cell sorting enables characterizing, defining and sorting of different cell types and subpopulations by analyzing the expression of cell-surface and intracellular molecules. Among some specific projects in which this instrument will be used are the determination of basic biological functions and signaling directed by cell surface proteins in stem cells, the role of endothelial cells on anti-apoptotic pathways, the regulation of pre-mRNA processing in plants, the effects of temperature in amphibian immunology, the development of tubular organs, and basic understanding of DNA repair mechanisms. Results from the research using the instrument will be disseminated in peer-reviewed publications, scientific meeting and to the community throughout outreach activities in which faculty of this institution participate.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.