The role of the N-end rule pathway in controlling plant response to the environment

操纵植物以在不可预测的生长条件下提供稳定的产量对于应对气候变化增加农业环境不确定性的影响至关重要。通过蛋白水解选择性和有条件地去除调节蛋白是植物中的主要调节原则。N-末端酶解项目的目的是提供一个完整的机制理解的N-末端规则的靶向蛋白水解途径在控制植物对环境的反应的作用。为了实现这一目标，选择了代表国际领先的欧洲团队的联盟合作伙伴，这些团队在该途径的多个方面具有互补的专业知识，包括该途径的生化基础，缺氧，应激和代谢信号的转录调控。该项目将汇集四个机构中在基础分子植物科学、生物化学和化学方面具有互补专长的六个研究小组。N-Cement联合体的研究方案将通过四个联合体伙伴（三个由ERA-CAPS资助，一个附属实验室）开展的六个相互关联的工作包（WP）来实现。这些工作包的目标是相关的三个基本问题，所产生的整合后，最近发现的财团成员的N-末端规则的有针对性的蛋白水解途径的作用，作为一个主要的调节植物发育和对环境的反应。问题1：N端规则途径的底物和酶组分是什么？问题2：N端规则通路如何整合到细胞信号通路中？问题3：N端规则途径在植物对生物和非生物环境的反应中的作用程度如何？这项研究具有高度创新的可测量成果，解决了植物生物学的这一新发现的领域，并将揭示：调节蛋白质稳定性的新机制，环境胁迫传感的新机制，蛋白质在胁迫传感中的新功能，N端规则在欧盟关键作物番茄中的重要性。这一快速发展的植物分子科学领域是由N-A成员领导的（包括底物的发现、进入N-末端规则途径的方法、途径的生化成分），加上联盟内大量独特资源的可用性，使得N-A提案非常新颖和及时。这一合作计划的协同价值将是开发和利用一个对农业至关重要的高度新颖的植物生物学领域，欧洲有能力通过ERA-CAPS资助在这一领域发挥世界领先作用。

Manipulation of plants to provide stability of yield under unpredictable growth conditions will be essential to respond to the effect of climate change in increasing the uncertainty of the agricultural environment. Selective and conditional removal of regulator proteins by proteolysis is emerging as a major regulatory principle in plants. The aim of the N-vironment project is to provide a complete mechanistic understanding of the role of the N-end rule pathway of targeted proteolysis in controlling plant responses to the environment. To achieve this goal consortium partners have been selected that represent internationally leading European teams with complementary expertise in the pathway´s multiple facets, including the biochemical basis of the pathway, hypoxia, transcriptional regulation of stress and metabolic signalling. The project will bring together six research groups with complementary expertise in fundamental molecular plant science, biochemistry and chemistry, in four institutions. The research programme of the N-vironment consortium will be achieved through six inter-related Work Packages (WPs) carried out by the four consortium partners (three funded by ERA-CAPS, one associated laboratory). The objectives of these work packages are related to three fundamental questions that arose after integration of the recent discoveries by consortium members of the role of the N-end rule pathway of targeted proteolysis as a major regulator of plant development and response to the environment. Question 1: What are the substrates and enzymatic components of the N-end rule pathway? Question 2: How is the N-end rule pathway integrated into cellular signaling pathways? Question 3: What is the extent of the role of the N-end rule pathway in plant response to the biotic and abiotic environment? The proposed research has highly innovative measurable outcomes that address this newly discovered area of plant biology, and will uncover: New mechanisms regulating protein stability, new mechanisms of environmental stress sensing, new functions for proteins in stress sensing, the importance of the N-end rule in a key EU crop, tomato. This fast-developing area of plant molecular science is led by N-vironment members (including discovery of substrates, methods of entry into the N-end rule pathway, biochemical components of the pathway), and together with the availability of a large number of unique resources within the consortium, makes the N-vironment proposal highly novel and timely. The synergistic value of this collaborative programme will be the development and exploitation of a highly novel area of plant biology of key importance to agriculture, in which Europe has the capacity to take a world lead through ERA-CAPS funding.