Taking a breather: Characterizing the role of H+/Ca transport in anoxia tolerance

植物经历动态环境，其中可能遇到许多胁迫条件。例如，洪水会引起几种压力：有限的氧气供应，温度变化，光线减少。缺氧，即氧气的去除，是植物被水淹没的结果。在这种压力下，植物组织显着降低能量生产率，影响代谢的许多方面。该项目研究了植物细胞内钙（Ca）的波动如何影响缺氧耐受性。研究人员发现，去除一种特定的钙转运蛋白似乎可以缓冲植物对缺氧条件的反应。在这里，成像实验将直观地解决这种变化如何影响钙的移动，它是本地化的，以及钙的时间和空间变化如何影响缺氧耐受性。为了补充这些摄影方法，生化和遗传实验将解决钙运动的机制。测量蛋白质和核酸变化的方法将用于进一步确定特定Ca转运蛋白的去除如何影响植物对能量的利用。这项工作的一个广泛影响将是与休斯顿社区学院（HCC）的教师和学生开展独特的研究合作。HCC是一所开放入学的公立学校，虽然服务于美国最多样化的人群之一，但通常不提供研究经验。这些HCC学生将学习并为这个项目做出贡献。这项工作的努力将导致更好地了解植物如何适应环境。未来，通过工程调控植物体内钙的迁移，可以规避缺氧条件的不利影响，减轻气候变化引起的作物损失。缺氧和缺氧是植物面临的严重生理胁迫。最常见的情况是，极端天气事件导致洪水泛滥，造成广泛的农作物损失。膜转运蛋白参与细胞信号转导和代谢过程的调控，是缺氧耐受的重要组成部分。拟南芥液泡阳离子/质子交换体（CAXs）的突变导致对缺氧的显著耐受。这种强大的表型是出乎意料的：功能丧失突变导致功能获得。大多数CAX RNA在缺氧期间没有变化，突出了RNA谱作为基因发现的唯一手段的局限性。一系列的遗传学、组学、膜转运、生理学、细胞和元素成像实验将用于表征CAX在缺氧期间的作用，这将证明它们在信号传导、阳离子转运和代谢中的作用。一个高技能，多元化的团队将使用多学科的方法，包括植物遗传学，膜运输和元素成像。作为更广泛的影响的一部分，并作为发现的进一步手段，休斯顿社区学院（HCC）将实施基于课程的本科生研究体验（CURE）。HCC是一所开放入学的公立学校，虽然为美国最多样化的人群之一提供服务，但通常不提供研究经验。简而言之，表征CAXs在缺氧耐受性中的作用将导致对植物信号转导的更好理解。此外，这些知识最终将有助于开发抗洪水的作物，减少极端天气事件期间的作物损失。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Plants experience a dynamic environment where numerous stress conditions may be encountered. For example, flooding induces several stresses: limited oxygen availability, changes in temperature, reduced light. Anoxia, the removal of oxygen, is a consequence of waterlogging and submergence of plants. During this stress, plant tissues significantly reduce the rate of energy production, affecting many aspects of metabolism. This project examines how fluctuations in calcium (Ca) within plant cells can impact anoxia tolerance. The investigators have discovered that removal of a specific Ca transporter appears to buffer the plant against anoxic conditions. Here imaging experiments will visually address how this change effects how Ca moves, where it is localized, and how the temporal and spatial changes in Ca can impact anoxia tolerance. To complement these photographic approaches, biochemical and genetic experiments will address the mechanisms of Ca movement. Approaches to measure changes in protein and nucleic acids will be used to further define how removal of a specific Ca transporter can impact the plant’s utilization of energy. One broad impact of this work will be the development of a unique research collaboration with faculty and students from Houston Community College (HCC). HCC is an open-admission, public school that while serving one of the most diverse populations in the US, does not usually provide research experiences. These HCC students will both learn and contribute to this project. The efforts of this work will lead to a better understanding of how plants adapt to their environment. In the future, engineering-modulated Ca movement in plants could circumvent adverse effects of anoxic conditions to mitigate climate change-induced crop loss.Oxygen deficiency (hypoxia) and absence (anoxia) are severe physiological stresses in plants. Most commonly they occur when extreme weather events lead to flooding, which causes widespread crop losses. Membrane transporters may be essential components of anoxia tolerance because they are involved in signal transduction and regulation of metabolic processes. Mutations in the Arabidopsis vacuolar cation/proton exchangers (CAXs) cause striking tolerance to anoxia. This robust phenotype was unexpected: a loss-of-function mutation causing a gain of function. The majority of CAX RNAs are unchanged during hypoxia, highlighting the limits of RNA profiling as an exclusive means of gene discovery. A series of genetic, omics, membrane transport, physiological, cell and elemental imaging experiments will be used to characterize the role of CAXs during anoxia that will demonstrate their role in signaling, cation transport and metabolism. A highly skilled, diverse team will use a multi-disciplinary approach including plant genetics, membrane transport and elemental imaging. As part of the broader impacts, and as a further means of discovery, a Course-based Undergraduate Research Experience (CURE) at Houston Community College (HCC) will be implemented. HCC is an open-admission public school that, while serving one of the most diverse populations in the US, does not typically provide research experiences. In short, characterizing the role of CAXs in anoxia tolerance will lead to a greater understanding of plant signal transduction. Furthermore, this knowledge will eventually aid in the development of flood-resistant crop plants, reducing crop loss during extreme weather events.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.